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Comparison of taurine chloramine and taurine bromamine effects on rheumatoid arthritis synoviocytes

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Summary.

Fibroblast-like synoviocytes (FLS) participate in rheumatoid arthritis (RA) chronic synovitis by producing pro-inflammatory cytokines (IL-6, IL-8), growth factors (VEGF) and other inflammatory mediators (PGE2, NO). We have previously reported that Tau-Cl, generated by neutrophils, inhibits in vitro some of these pathogenic RA FLS functions. Taurine bromamine (Tau-Br) originates from eosinophils and neutrophils, and its immunoregulatory activities are poorly known. Therefore, we investigated the effects of Tau-Br on RA FLS functions and compared it to Tau-Cl anti-inflammatory action. When applied at noncytotoxic concentrations: (i) Tau-Br inhibited IL-6 and PGE2 production with potency similar to Tau-Cl (IC50 ≈ 250 µM), (ii) Tau-Br failed to affect VEGF and IL-8 synthesis, while Tau-Cl exerted inhibitory effect (IC50 ≈ 400 µM), (iii) none of these compounds affected NO generation and iNOS expression. Thus, Tau-Cl is more effective than Tau-Br in normalization of pro-inflammatory RA FLS functions.

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Authors and Affiliations

  1. Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland

    E. Kontny, M. Chorąży-Massalska, W. Rudnicka & W. Maśliński

  2. Department of Immunology, Jagiellonian University Medical College, Cracow, Poland

    J. Marcinkiewicz

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  1. E. Kontny
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  2. M. Chorąży-Massalska
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  3. W. Rudnicka
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  4. J. Marcinkiewicz
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  5. W. Maśliński
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Kontny, E., Chorąży-Massalska, M., Rudnicka, W. et al. Comparison of taurine chloramine and taurine bromamine effects on rheumatoid arthritis synoviocytes. Amino Acids 32, 447–452 (2007). https://doi.org/10.1007/s00726-006-0368-0

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