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Proteomic analysis of the cerebrospinal fluid of patients with schizophrenia

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Summary.

 We applied proteomics technologies to analyze the cerebrospinal fluid of patients with schizophrenia. Such an analysis can result in the identification of proteins, which may play a role in the disease progress and thus lead to the discovery of clues of the etiology of schizophrenia. Cerebrospinal fluid from patients and controls was analyzed by two-dimensional gels and the proteins were identified by matrix-assisted laser desorption ionization mass spectrometry (MS) in the MS and MS/MS mode. 54 different gene products were identified, which were mainly plasma proteins. The level of apolipoprotein A-IV was significantly decreased in the schizophrenic patients compared to that in the controls. Little is known about the function of this apolipoprotein in the central nervous system. The levels of certain other proteins, like haptoglobin, fibrinogen, complement component 3, and Gc-globulin, were altered in the disease group as well, however, the changes did not reach a statistical significance.

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Authors and Affiliations

  1.  Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai, China, CN

    L. Jiang & L. He

  2.  F. Hoffmann-La Roche Ltd., Center for Medical Genomics, Basel, Switzerland, CH

    K. Lindpaintner, H. Langen & M. Fountoulakis

  3.  Shanghai Institute of Mental Health, 600 South Wan Ping Road, Shanghai, China, CN

    H.-F. Li & N.-F. Gu

Authors
  1. L. Jiang
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  2. K. Lindpaintner
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  3. H.-F. Li
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  4. N.-F. Gu
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  5. H. Langen
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  6. L. He
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  7. M. Fountoulakis
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Additional information

Acknowledgements We thank J.-F. Juranville and D. Avila for technical assistance. The sample collection were supported by grants from the national 973 and 863 Projects, the National Natural Science Foundation of China and Shanghai Municipal Commission for Science and Technology. The research fund was from F. Hoffmann-La Roche Ltd.

Authors' address: Michael Fountoulakis, F. Hoffmann-La Roche Ltd, Center for Medical Genomics, Building 93-444, CH-4070 Basel, Switzerland, or Lin He, Shanghai Jiao Tong University, Bio-X Life Science Research Center, Hao Ran Building, PO Box 501, 1954 Hua Shan Road, Shanghai 200030, P.R. China, Fax: 86-21-62822491, E-mail: helin@sjtu.edu.cn

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Jiang, L., Lindpaintner, K., Li, HF. et al. Proteomic analysis of the cerebrospinal fluid of patients with schizophrenia. Amino Acids 25, 49–57 (2003). https://doi.org/10.1007/s00726-003-0356-6

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  • DOI: https://doi.org/10.1007/s00726-003-0356-6

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