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Evaluation of a Shuttle DNP Spectrometer by Calculating the Coupling and Global Enhancement Factors of l-Tryptophan

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Abstract

A liquid state shuttle dynamic nuclear polarization (DNP) spectrometer is presented, featuring several technical modifications that increase stability and improve reproducibility. For the protons of l-tryptophan, the signal enhancement and the DNP spin properties, such as relaxation, were measured and compared with each other. The calculated coupling factors suggest that the proton accessibility for the polarizer molecule has an important influence on the DNP enhancement. In general, short proton spin longitudinal relaxation times without radical reduce the detectable enhancement by decreasing the leakage factor and increasing the relaxation losses during the course of the sample transfer. The usage of a global enhancement factor gives a more complete overview of the capabilities for the described experimental setup. Global enhancements of up to −4.2 for l-tryptophan protons are found compared to pure Boltzmann enhancements of up to −2.4.

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Notes

  1. Note that our cavity has a complex inner contour, which distorts the modal field distribution such that the classification in classical TM and TE modes becomes ambiguous. However, due to the close similarity of the distorted modal field to that of classical cavity modes we believe it is justified to retain the classical nomenclature.

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Acknowledgments

We would like to acknowledge M.T. Türke for helpful discussion and T. Michael Sabo for carefully reading the manuscript. This work was supported by the Max Planck Society (to M.B. and C.G.) and by Bio-NMR project 261863 (to C.G. and F.E.).

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Authors and Affiliations

  1. NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany

    Philip Lottmann & Christian Griesinger

  2. Bruker BioSpin, Silberstreifen 4, 76287, Rheinstetten, Germany

    Thorsten Marquardsen, Alexander Krahn, Andreas Tavernier, Peter Höfer & Frank Engelke

  3. Electron Paramagnetic Resonance, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany

    Marina Bennati

Authors
  1. Philip Lottmann
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  2. Thorsten Marquardsen
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  3. Alexander Krahn
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  4. Andreas Tavernier
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  5. Peter Höfer
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  6. Marina Bennati
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  7. Frank Engelke
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  8. Christian Griesinger
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Correspondence to Frank Engelke or Christian Griesinger.

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Lottmann, P., Marquardsen, T., Krahn, A. et al. Evaluation of a Shuttle DNP Spectrometer by Calculating the Coupling and Global Enhancement Factors of l-Tryptophan. Appl Magn Reson 43, 207–221 (2012). https://doi.org/10.1007/s00723-012-0345-6

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  • DOI: https://doi.org/10.1007/s00723-012-0345-6

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