Abstract
Several eukaryotic cell lineages proliferate by multiple fission cell cycles, during which cells grow to manyfold of their original size, then undergo several rounds of cell division without intervening growth. A previous study on volvocine green algae, including both unicellular and multicellular (colonial) species, showed a correlation between the minimum number of successive cell divisions without intervening cellular growth, and the threshold cell size for commitment to the first round of successive cell divisions: two times the average newly born daughter cell volume for unicellular Chlamydomonas reinhardtii, four times for four-celled Tetrabaena socialis, in which each cell in the colony produces a daughter colony by two successive cell divisions, and eight times for the eight-celled Gonium pectorale, in which each cell produces a daughter colony by three successive cell divisions. To assess whether this phenomenon is also applicable to other lineages, we have characterized cyanidialean red algae, namely, Cyanidioschyzon merolae, which proliferates by binary fission, as well as Cyanidium caldarium and Galdieria sulphuraria, which form up to four and 32 daughter cells (autospores), respectively, in a mother cell before hatching out. The result shows that there is also a correlation between the number of successive cell divisions and the threshold cell size for cell division or the first round of the successive cell divisions. In both C. merolae and C. caldarium, the cell size checkpoint for cell division(s) exists in the G1-phase, as previously shown in volvocine green algae. When C. merolae cells were arrested in the G1-phase and abnormally enlarged by conditional depletion of CDKA, the cells underwent two or more successive cell divisions without intervening cellular growth after recovery of CDKA, similarly to C. caldarium and G. sulphuraria. These results suggest that the threshold size for cell division is a major factor in determining the number of successive cell divisions and that evolutionary changes in the mechanism of cell size monitoring resulted in a variation of multiple fission cell cycle in eukaryotic algae.
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We thank members of the SyM. Laboratory for their technical advice and support.
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This study was supported by grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (nos. 17H01446 and 20H00477 to SM and no. 19J13366 to LWJ) and the JST-MIRAI Program of Japan Science and Technology Agency (JST) (to SyM).
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L.W.J., T.K., and Sy.M. designed the study. L.W.J. performed experiments. T.K. and S.H. provided strains and information on the cultivation of the strains. L.W.J., T.K., S.H., and Sy.M. analyzed and interpreted the data. L.W.J. and Sy.M. wrote the manuscript.
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Jong, L.W., Fujiwara, T., Hirooka, S. et al. Cell size for commitment to cell division and number of successive cell divisions in cyanidialean red algae. Protoplasma 258, 1103–1118 (2021). https://doi.org/10.1007/s00709-021-01628-y
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DOI: https://doi.org/10.1007/s00709-021-01628-y