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Rice terpene synthase 20 (OsTPS20) plays an important role in producing terpene volatiles in response to abiotic stresses

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Abstract

This study examined the volatile terpenes produced by rice seedlings in response to oxidative stress induced by various abiotic factors. Solid-phase microextraction–gas chromatography–mass spectrometry (SPME–GC–MS) analyses revealed that when exposed to UV-B radiation, rice seedlings emitted a bouquet of monoterpene mixtures in a time-dependent manner. The mixtures comprised limonene, sabinene, myrcene, α-terpinene, β-ocimene, γ-terpinene, and α-terpinolene. Among them, (S)-limonene was the most abundant volatile, discriminated by chiral SPME–GC–MS. The volatile profiles collected from rice plants treated with both γ-irradiation and H2O2 were identical to those observed in the UV-B irradiated plants, thus indicating that the volatile mixtures were specifically produced in response to oxidative stress, particularly in the presence of H2O2. Using a reverse genetics approach, we isolated full-length rice terpene synthase 20 (OsTPS20, 599 amino acids, 69.39 kDa), which was further characterized as an (S)-limonene synthase by removing the N-terminal signal peptide (63 amino acids) of the protein. The recombinant OsTPS20 protein catalyzed the conversion of geranyl diphosphate to (S)-limonene and other minor monoterpenes, essentially covering all of the volatile compounds detected from the plant. Moreover, qRT-PCR revealed that the transcript levels of OsTPS20 were significantly induced in response to oxidative stress, thereby suggesting that OsTPS20 plays a major role in producing terpene volatiles during abiotic stress. Detailed biochemical analyses and the unusual domain characteristics of OsTPS20 are also discussed in this report.

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Acknowledgments

This research was supported by the Nuclear R & D Program of the Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do, 580-185, Republic of Korea

    Gun Woong Lee, Sungbeom Lee, Moon-Soo Chung, Yeon Sim Jeong & Byung Yeoup Chung

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  1. Gun Woong Lee
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  2. Sungbeom Lee
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Correspondence to Byung Yeoup Chung.

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Handling Editor: Peter Nick

Gun Woong Lee and Sungbeom Lee contributed equally to this study.

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Lee, G.W., Lee, S., Chung, MS. et al. Rice terpene synthase 20 (OsTPS20) plays an important role in producing terpene volatiles in response to abiotic stresses. Protoplasma 252, 997–1007 (2015). https://doi.org/10.1007/s00709-014-0735-8

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  • DOI: https://doi.org/10.1007/s00709-014-0735-8

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