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Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice

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Abstract

The plant hormone jasmonic acid (JA) regulates various developmental processes and plant defence responses to environmental stresses. We previously reported that RERJ1, a JA-inducible transcription factor in rice, is up-regulated by exposure to wounding and drought stress. Here, we demonstrated that the expression of RERJ1 after wounding is regulated in a JA-dependent manner in rice, based on histochemical analysis of RERJ1 promoter-GUS transgenic plants. RERJ1 expression was induced only at the region of injury after wounding, whereas expression was induced in the entire leaf after drought. According to JA measurements of stressed leaves, high accumulation of endogenous JA was only detected around the wound site in a rice leaves, whereas the drought treatment led to uniform accumulation of JA in the entire leaf, suggesting that RERJ1will be a useful marker gene for studies on localization of JA in rice. Nuclear localization and transactivation ability of RERJ1 were also demonstrated. These results suggest that RERJ1 plays a role as a transcriptional activator for regulating stress-inducible gene expression, with a strong correlation to JA accumulation in the stressed region.

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Abbreviations

JA:

Jasmonic acid

bHLH:

Basic helix–loop–helix

DMSO:

Dimethyl sulphoxide

qRT-PCR:

Quantitative reverse-transcription-polymerase chain reaction

GFP:

Green fluorescent protein

LC-ESI-MS/MS:

Liquid chromatography-electrospray ionization/tandem mass spectrometry

CaMV:

Cauliflower mosaic virus

LUC:

Luciferase

ABA:

Abscisic acid

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Acknowledgments

We thank Professor Peter Nick (Karlsruhe Institute of Technology) for providing seeds of the hebiba mutant; Professor Tsuyoshi Nakagawa (Shimane University) for providing pGWB3, pGWB4, and pGWB5; and Dr M. Takagi and Dr M. Shikata (National Institute of Advanced Industrial Science and Technology) for providing 35S-GAL4DB, GAL4-TATA-LUC-NOS and pPTRL plasmids. This work was supported in part by Grants-in-Aid for Scientific Research (Researcher Number: 20312241) from the Japan Society for the Promotion of Science, and the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Koji Miyamoto, Takafumi Shimizu, Hideaki Nojiri, Hisakazu Yamane & Kazunori Okada

  2. Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Susumu Mochizuki, Yoko Nishizawa & Eiichi Minami

  3. Japan Society for the Promotion of Science, Tokyo, Japan

    Koji Miyamoto

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  1. Koji Miyamoto
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  2. Takafumi Shimizu
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  3. Susumu Mochizuki
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  4. Yoko Nishizawa
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  5. Eiichi Minami
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  6. Hideaki Nojiri
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  7. Hisakazu Yamane
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  8. Kazunori Okada
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Correspondence to Kazunori Okada.

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Handling Editor: Peter Nick

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Miyamoto, K., Shimizu, T., Mochizuki, S. et al. Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice. Protoplasma 250, 241–249 (2013). https://doi.org/10.1007/s00709-012-0400-z

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  • DOI: https://doi.org/10.1007/s00709-012-0400-z

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