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Constitutive expression of high-affinity sulfate transporter (HAST) gene in Indian mustard showed enhanced sulfur uptake and assimilation

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Abstract

Lycopersicon esculantum sulfate transporter gene (LeST 1.1) encodes a high-affinity sulfate transporter (HAST) located in root epidermis. In this study, the LeST 1.1 gene was constitutively expressed in Indian mustard (Brassica juncea cv. Pusa Jai Kisan). Transgenic as well as untransformed plants were grown in sulfur-insufficient (25 and 50 μM) and sulfur-sufficient (1,000 μM) conditions for 30 days. Two-fold increase was noticed in the sulfate uptake rate of transgenic plants grown in both sulfur-insufficient and -sufficient conditions as compared to untransformed plants. The transgenic B. juncea plants were able to accumulate higher biomass and showed improved sulfur status even in sulfur-insufficient conditions when compared with untransformed plants. Chlorophyll content, ATP sulfurylase activity and protein content were also higher in transgenic plants than untranformed plants under sulfur-insufficient conditions. Our results, thus, clearly indicate that constitutive expression of LeST 1.1 gene in B. juncea had led to enhanced capacity of sulfur uptake and assimilation even in sulfur-insufficient conditions. This approach can also be used in other crops to enhance their sulfate uptake and assimilation potential under S-insufficient conditions.

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Acknowledgements

We thank Dr. M. J. Hawkesford (Crop Performance and Improvement Division, Rothamsted Research Harpenden Herts, UK, AL52JQ) for the gift of pBin 19-LeST 1.1cDNA construct. One of the authors (M. Akmal) is thankful to the Council of Scientific and Industrial Research for the award of research fellowship for his Doctoral research. We are also thankful to Dr. M. A. A. Khan, NISCAIR, New Delhi, for editing the manuscript.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India

    M. Z. Abdin, M. Akmal, M. Ram, T. Nafis, P. Alam, M. Nadeem & M. A. Khan

  2. Department of Botany, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India

    A. Ahmad

  3. Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, India

    M. Z. Abdin

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  1. M. Z. Abdin
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Correspondence to M. Z. Abdin.

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Handling Editor: Bhumi Nath Tripathi

Authors M. Z. Abdin and M. Akmal contributed equally to this work.

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Abdin, M.Z., Akmal, M., Ram, M. et al. Constitutive expression of high-affinity sulfate transporter (HAST) gene in Indian mustard showed enhanced sulfur uptake and assimilation. Protoplasma 248, 591–600 (2011). https://doi.org/10.1007/s00709-010-0216-7

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