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The reaction of Lupinus angustifolius L. root meristematic cell nucleoli to lead

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Abstract

The effect of 2–48 h treatment of Lupinus angustifolius L. roots with lead nitrate at the concentration of 10−4 M on the nucleoli in meristematic cells was investigated. In the lead presence the number of ring-shaped as well as segregated nucleoli increased especially after 12–48 h of treatment, while spindle-shaped nucleoli appeared after 24 h and 48 h. Lead presence also increased the frequency of cells with silver-stained particles in the nucleus and the number of these particles especially from the 12th hour of treatment. It was accompanied by significant decline of nucleolar area. Analysis of these cells in transmission electron microscope confirmed the presence of ring-shaped and segregated nucleoli. Moreover, electron microscopy revealed compact structure nucleoli without granular component. Additionally, one to three oval-shaped fibrillar structures attached to nucleolus or lying free in the nucleoplasm were visible. The possible mechanism of lead toxicity to the nucleolus is briefly discussed.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Plant Cytology and Cytochemistry, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland

    Łucja Balcerzak & Mirosław Godlewski

  2. Laboratory of Electron Microscopy, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland

    Sława Glińska

Authors
  1. Łucja Balcerzak
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  2. Sława Glińska
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  3. Mirosław Godlewski
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Correspondence to Łucja Balcerzak.

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Handling Editor: Bhumi Nath Tripathi

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Balcerzak, Ł., Glińska, S. & Godlewski, M. The reaction of Lupinus angustifolius L. root meristematic cell nucleoli to lead. Protoplasma 248, 353–361 (2011). https://doi.org/10.1007/s00709-010-0177-x

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  • DOI: https://doi.org/10.1007/s00709-010-0177-x

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