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Development of mucilage cells of Araucaria angustifolia (Araucariaceae)

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Summary.

The roles of mucilage cells were investigated through morphological and cytological analysis during leaf development in young Araucaria angustifolia plants. Differentiation began in leaf primordia in the shoot apex, when the young cells underwent a greater increase in volume in comparison with other mesophyll cells. The mucilage polysaccharides were synthesized by dictyosomes, from where they were taken by large vesicles and released into a cavity formed by detachment of the tonoplast, which was separated from the cytoplasm. At the end of differentiation, the cell was completely filled with mucilage, a gel consisting of a denser reticular structure surrounding less dense regions. The nucleus and cytoplasm were degenerated in mature cells. The A. angustifolia mucilage cells presented some cytological resemblances to the mucilage cells of members of some dicotyledonous families; however, differences in the dictyosomes and the secretion route were observed. Translocation and water storage of solutes was suggested by the use of the hydroxy pyrenetrisulfonic acid tri-sodium salt apoplastic tracer. The tonoplast detachment, dechromatinization, nuclear condensation, and general degeneration of the membrane systems observed during maturity indicated a programmed cell death process, one not yet described for angiosperm mucilage cells.

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Abbreviations

HTPS:

hydroxy pyrenetrisulfonic acid tri-sodium salt

PCD:

programmed cell death

LM:

light microscopy

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Departamento de Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul

    A. A. Mastroberti & J. E. de Araujo Mariath

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  1. A. A. Mastroberti
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  2. J. E. de Araujo Mariath
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Correspondence to A. A. Mastroberti.

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Correspondence: A. A. Mastroberti, Departamento de Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Prédio 43423 sala 204, Porto Alegre, Rio Grande do Sul, CEP 91501-970, Brazil.

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Mastroberti, A., de Araujo Mariath, J. Development of mucilage cells of Araucaria angustifolia (Araucariaceae). Protoplasma 232, 233–245 (2008). https://doi.org/10.1007/s00709-007-0274-7

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