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Localization of arabinogalactan proteins in anther, pollen, and pollen tube of Nicotiana tabacum L.

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Summary.

Western blot analysis indicated the presence of two epitopes recognized by the anti-arabinogalactan protein antibodies JIM13 and LM2 and the absence of the JIM4 epitope in mature tobacco anthers. Immunoenzyme localization of arabinogalactan proteins (AGPs) with JIM13 showed that AGPs accumulate mainly at the early stages of anther development. AGP content and distribution were also investigated at the ultrastructural level in pollen tubes grown in vivo and in vitro. Abundant AGPs were present in the transmitting tissue of styles, and the AGP content of the extracellular matrix changed during pollen tube growth. In pollen tubes, immunogold particles were mainly distributed in the cell wall and cytoplasm, especially around the peripheral region of the generative-cell wall. β-D-Glucosyl Yariv reagent, which specifically binds to AGPs, caused slow growth of pollen tubes and reduced immunogold labeling of AGPs with JIM13 in vitro. These data suggest that AGPs participate in male gametogenesis and pollen tube growth and may be important surface molecules in generative and sperm cells.

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Abbreviations

AGPs:

arabinogalactan proteins

ECM:

extracellular matrix

GM:

germination medium

MAB:

monoclonal antibody

TT:

transmitting tissue

βGlcY:

β-D-glucosyl Yariv reagent

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan

    Y. Qin, D. Chen & J. Zhao

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  1. Y. Qin
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  2. D. Chen
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  3. J. Zhao
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Correspondence to J. Zhao.

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Correspondence and reprints: Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, People’s Republic of China.

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Qin, Y., Chen, D. & Zhao, J. Localization of arabinogalactan proteins in anther, pollen, and pollen tube of Nicotiana tabacum L.. Protoplasma 231, 43–53 (2007). https://doi.org/10.1007/s00709-007-0245-z

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  • DOI: https://doi.org/10.1007/s00709-007-0245-z

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