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Effect of CO2 supply on formation of reactive oxygen species in Arabidopsis thaliana

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Summary.

Light-induced generation of reactive oxygen species (ROS) in 2-week-old leaves of Arabidopsis thaliana was studied by means of the ROS-sensitive dyes nitroblue tetrazolium (NBT) and 5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA). Superposition of pictures of chlorophyll fluorescence and DCF fluorescence indicated that the origin of ROS was in the chloroplasts. Experiments were done with zero, 0.1, or 10 mM NaHCO3 in the infiltration medium. Energy quenching in photosystem II was higher under low CO2 concentrations as measured by chlorophyll fluorescence. DCF fluorescence showed that CO2 deficiency led to an increase of ROS generation. In contrast, the photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea reduced the light-induced increase of DCF fluorescence. This indicates that ROS production does not primarily result from over-reduction of photosystem II as caused by impeding electron flow in the electron transfer chain. More likely, it is an effect of diverting electron flux normally aimed at carboxylation in the Calvin cycle to other sinks more prone to the generation of toxic radicals. There was no significant effect of salicyl hydroxamate (a blocker of the alternative oxidase), showing that the mitochondrial electron transfer chain seems to play a minor role as already indicated by the superposition of chlorophyll and DCF fluorescence.

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl-)1,1-dimethylurea

DCF-DA:

5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate

NBT:

nitroblue tetrazolium

ROS:

reactive oxygen species

SHAM:

salicyl hydroxamate

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Authors and Affiliations

  1. Center of Biochemistry and Molecular Biology, Christian-Albrechts-Universität zu Kiel, Kiel

    A. Hoffmann, E. Hammes, C. Plieth & U.-P. Hansen

  2. Institute of Plant Nutrition and Soil Science, Christian-Albrechts-Universität zu Kiel, Kiel

    B. Sattelmacher

  3. Institute of Botany, Christian-Albrechts-Universität zu Kiel, Kiel

    C. Desel

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  1. A. Hoffmann
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  2. E. Hammes
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  3. C. Plieth
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  4. C. Desel
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  5. B. Sattelmacher
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  6. U.-P. Hansen
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Correspondence to U.-P. Hansen.

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Correspondence and reprints: Center of Biochemistry and Molecular Biology, Christian-Albrechts-Universität zu Kiel, Leibnizstrasse 11, 24098 Kiel, Federal Republic of Germany.

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Hoffmann, A., Hammes, E., Plieth, C. et al. Effect of CO2 supply on formation of reactive oxygen species in Arabidopsis thaliana. Protoplasma 227, 3–9 (2005). https://doi.org/10.1007/s00709-005-0133-3

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