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Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth

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Summary.

Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiology. Despite the intriguing similarities between animals and plants in NAE metabolism and perception, not much is known about the precise function of these metabolites in plant physiology. In plants, NAEs have been shown to inhibit phospholipase Dα (PLDα) activity, interfere with abscisic acid-induced stomatal closure, and retard Arabidopsis seedling development. 1-Butanol, an antagonist of PLD-dependent phosphatidic acid production, was reported to induce defects in Arabidopsis seedling development that were somewhat similar to effects induced by elevated levels of NAE. This raised the possibility that the impact of NAE on seedling growth could be mediated in part via its influence on PLD activity. To begin to address this possibility, we conducted a detailed, comparative analysis of the effects of 1-butanol and N-lauroylethanolamine (NAE 12:0) on Arabidopsis root cell division, in vivo cytoskeletal organization, seed germination, and seedling growth. Although both NAE 12:0 and 1-butanol induced profound cytoskeletal and morphological alterations in seedlings, there were distinct differences in their overall effects. 1-Butanol induced more pronounced modifications in cytoskeletal organization, seedling growth, and cell division at concentrations severalfold higher than NAE 12:0. We propose that these compounds mediate their differential effects on cellular organization and seedling growth, in part through the differential modulation of specific PLD isoforms.

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Abbreviations

GFP:

green-fluorescent protein

LA:

lauric acid

NAE:

N-acylethanolamine

NAPE:

N-acylphosphatidylethanolamine

PLD:

phospholipase D

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Authors and Affiliations

  1. Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma

    Christy M. Motes, Priit Pechter, Cheol Min Yoo, Yuh-Shuh Wang & Elison B. Blancaflor

  2. Center for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, Texas

    Kent D. Chapman

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  1. Christy M. Motes
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  2. Priit Pechter
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  3. Cheol Min Yoo
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  4. Yuh-Shuh Wang
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  5. Kent D. Chapman
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  6. Elison B. Blancaflor
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Correspondence to Elison B. Blancaflor.

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Correspondence and reprints: Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, U.S.A.

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Motes, C., Pechter, P., Yoo, C. et al. Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth. Protoplasma 226, 109–123 (2005). https://doi.org/10.1007/s00709-005-0124-4

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