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Superoxide dismutase, peroxidase, and germin-like protein activity in plasma membranes and apoplast of maize roots

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Summary.

The analysis of plasma membranes from maize roots by native gel electrophoresis revealed the existence of Mn-containing 120 kDa and CuZn-containing 70, 40, and 15 kDa superoxide dismutase (SOD) isoform activities. Isoelectric focusing of the plasma membranes differentiated anionic SOD isoforms with a pI of about 5 and cationic SOD isoforms at pI 8.6. Solubilization of the plasma membrane proteins further separated the cationic SOD into pI 8.6, 8.2, 8.4, and 7.2 isoforms. Double staining for both SOD and peroxidase activities showed an overlap of these activities only in the case of the high-molecular-mass (ca. 120 kDa) isoforms. High-temperature treatments demonstrated that the 120 kDa isoform was active even at 100 °C, indicating that it was a germin-like protein with superoxide-dismutating activity, different from the peroxidase with a similar molecular mass and the lower-molecular-mass CuZn-containing superoxide dismutases. These results are compared to those obtained from whole-tissue extract and apoplastic fluid.

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Authors and Affiliations

  1. Center for Multidisciplinary Studies, Belgrade University, Belgrade

    B. Kukavica & Ž. Vučinić

  2. Maize Research Institute, Belgrade

    M. Vuletić

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  1. B. Kukavica
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  2. Ž. Vučinić
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  3. M. Vuletić
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Correspondence to M. Vuletić.

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Correspondence and reprints: Maize Research Institute, POB 89-Zemun, 11081 Belgrade, Serbia and Montenegro.

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Kukavica, B., Vučinić, Ž. & Vuletić, M. Superoxide dismutase, peroxidase, and germin-like protein activity in plasma membranes and apoplast of maize roots. Protoplasma 226, 191–197 (2005). https://doi.org/10.1007/s00709-005-0112-8

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