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Aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of Triticum turgidum

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Summary.

The effects of aluminium on the actin filament (AF) cytoskeleton of Triticum turgidum meristematic root tip cells were examined. In short treatments (up to 2 h) with 50–1000 μM AlCl3·6H2O, interphase cells displayed numerous AFs arrayed in thick bundles that lined the plasmalemma and traversed the endoplasm in different directions. Measurements using digital image analysis and assessment of the overall AF fluorescence revealed that, in short treatments, the affected cells possessed 25–30% more AFs than the untreated ones. The thick AF bundles were not formed in the Al-treated cells in the presence of the myosin inhibitors 2,3-butanedione monoxime (BDM) and 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine (ML-7), a fact suggesting that myosins are involved in AF bundling. In longer Al treatments, the AF bundles were disorganised, forming granular actin accumulations, a process that was completed after 4 h of treatment. In the Al-treated cells, increased amounts of callose were uniformly deposited along the whole surface of the cell walls. In contrast, callose formed local deposits in the Al-treated cells in the presence of cytochalasin B, BDM, or ML-7. These results favour the hypothesis that the actomyosin system in the Al-treated cells, among other roles, participates in the mechanism controlling callose deposition.

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Abbreviations

AF:

actin filament

BDM:

2,3-butanedione monoxime

CB:

cytochalasin B

ML-7:

1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine

MT:

microtubule

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  1. Department of Botany, Faculty of Biology, University of Athens, Athens

    G. Frantzios, B. Galatis & P. Apostolakos

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  1. G. Frantzios
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  2. B. Galatis
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  3. P. Apostolakos
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Correspondence to P. Apostolakos.

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Correspondence and reprints: Department of Botany, Faculty of Biology, University of Athens, Athens 157 84, Greece.

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Frantzios, G., Galatis, B. & Apostolakos, P. Aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of Triticum turgidum. Protoplasma 225, 129–140 (2005). https://doi.org/10.1007/s00709-005-0100-z

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