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Authentication of senna extract from the eighteenth century and study of its composition by HPLC–MS

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Abstract

The historical relic of medicinal preparation of senna extract, over 200 years old, was analyzed using RP-HPLC connected with ESI−-MSn. The conditions for extracting the compounds from the sample and separating them by HPLC were optimized. A broad spectrum of glycosides was identified. Both main senna anthraquinone glycosides, sennoside A and sennoside B, were not detected in the sample. Nevertheless, the found presence of other substances typical of senna allows the positive authentication of the sample. Three possible degradation products of sennosides were identified; rhein and two compounds with unresolved structure. Remarkable stability of some glycosides in the historical sample was found. Detailed ESI−-MSn fragmentation mechanisms of sennoside A and B have been proposed.

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Acknowledgements

The financial support by the projects SVV 260560 and Progress Q46 of Charles University is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic

    Karel Nesměrák, Karel Kudláček, Peter Čambal, Petr Kozlík & Václav Červený

  2. Department of Chemistry, Faculty of Science, Charles University, Prague, Czech Republic

    Martin Štícha

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  1. Karel Nesměrák
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  2. Karel Kudláček
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  3. Peter Čambal
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  4. Martin Štícha
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  5. Petr Kozlík
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  6. Václav Červený
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Correspondence to Karel Nesměrák.

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Nesměrák, K., Kudláček, K., Čambal, P. et al. Authentication of senna extract from the eighteenth century and study of its composition by HPLC–MS. Monatsh Chem 151, 1241–1248 (2020). https://doi.org/10.1007/s00706-020-02630-5

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  • DOI: https://doi.org/10.1007/s00706-020-02630-5

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