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Immobilization of horseradish peroxidase on zinc oxide nanorods grown directly on electrodes for hydrogen peroxide sensing

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Abstract

An amperometric biosensor for hydrogen peroxide detection was developed by immobilization of horseradish peroxidase on zinc oxide nanorods directly grown on the electrode. The ZnO nanorods were fabricated by electrodepositing ZnO nanoseeds on indium tin oxide glass followed by a hydrothermal growth process. The ZnO nanorods were used to increase the specific surface area for carrying horseradish peroxidase and to build direct electron transfer channels to the electrode. The structure of the ZnO nanorods on indium tin oxide was studied by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and the electrochemical behaviors were carefully examined. The result showed that the biosensor responds well to hydrogen peroxide in a wide range of concentration. The linear range is from 0.5 to 9.0 mM with a limit of detection (S/N = 3) of 0.3 mM, and the response time is about 7 s, which indicates that ZnO nanorods are promising supporting materials to build direct electron transfer biosensors.

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References

  1. Wei A, Sun XW, Wang JX, Lei Y, Cai XP (2006) Appl Phys Lett 89:123902

    Article  Google Scholar 

  2. Ahmad M, Pan CF, Gan L, Nawaz Z, Zhu J (2010) J Phys Chem C 114:243

    Article  CAS  Google Scholar 

  3. Chen ZZ, Ren XL, Meng XW, Chen D, Yan CM, Ren J, Yuan Y, Tanga FQ (2011) Biosens Bioelectron 28:50

    Article  Google Scholar 

  4. Jia JB, Wang BQ, Wu AG, Cheng GJ, Li Z, Dong SJ (2002) Anal Chem 74:2217

    Article  CAS  Google Scholar 

  5. Dai ZH, Shao GJ, Hong JM, Bao JC, Shen J (2009) Biosens Bioelectron 24:1286

    Article  CAS  Google Scholar 

  6. Kong T, Chen Y, Ye YP, Zhang K, Wang ZX, Wang XP (2009) Sens Actuators B 138:344

    Article  CAS  Google Scholar 

  7. Yang Z, Ye ZZ, Zhao BH, Zong XL, Wang P (2010) Physica E 42:1830

    Article  CAS  Google Scholar 

  8. Zhang XM, Lu MY, Zhang Y, Chen LJ, Wang ZL (2009) Adv Mater 21:2767

    Article  CAS  Google Scholar 

  9. Wang JX, Sun XW, Wei A, Lei Y, Cai XP, Li CM, Dong ZL (2006) Appl Phys Lett 88:233106

    Article  Google Scholar 

  10. Zhu X, Yuri I, Gan X, Suzuki I, Li G (2007) Biosens Bioelectron 22:1600

    Article  CAS  Google Scholar 

  11. Fan Z, Lu JG (2005) Appl Phys Lett 86:032111

    Article  Google Scholar 

  12. Xu CX, Sun XW, Yuen CB, Chen J, Yu SF, Dong ZL (2005) Appl Phys Lett 86:011118

    Article  Google Scholar 

  13. Kumar S, Gupta V, Sreenivas K (2006) Nanotechnology 16:1167

    Article  Google Scholar 

  14. Liu X, Hu Q, Wu Q, Zhang W, Fang Z, Xie Q (2009) Colloids Surf B 74:154

    Article  CAS  Google Scholar 

  15. Liu JP, Guo CX, Li CM, Li YY, Huang XT, Liao L, Yu T (2009) Electrochem Comm 11:202

    Article  CAS  Google Scholar 

  16. Gu BX, Xu CX, Zhu GP, Liu SQ, Chen LY, Li XS (2009) J Phys Chem B 113:377

    Article  CAS  Google Scholar 

  17. Umar A, Rahman MM, Al-Hajry A, Hahn YB (2009) Talanta 78:284

    Article  CAS  Google Scholar 

  18. Umar A, Rahman MM (2009) J Nanosci Nanotechnol 9:4686

    Article  CAS  Google Scholar 

  19. Tian ZT, Voigt JA, Liu J, McKenzie B, McDermott MJ, Rodriguez MA, Konishi H, Xu H (2003) Nat Mater 29:821

    Article  Google Scholar 

  20. Vayssieres L (2003) Adv Mater 15:464

    Article  CAS  Google Scholar 

  21. Zunger A, Wagner S, Petroff PM (1993) J Electron Mater 22:1

    Article  Google Scholar 

  22. Chemseddine A (1994) J Sol-Gel Science Technol 2:685

    Article  CAS  Google Scholar 

  23. Liao QL, Yang Y, Xia LS, Qi JJ, Zhang Y, Huang YH, Qin Z (2008) Phys Plasmas 15:114505

    Article  Google Scholar 

  24. Gu BX, Xu CX, Zhu GP, Liu SQ, Chen LY, Wang ML, Zhu JJ (2009) J Phys Chem B 113:6553

    Article  CAS  Google Scholar 

  25. Rubinow SI, Lebowitz JL (1970) J Am Chem Soc 92:3888

    Article  CAS  Google Scholar 

  26. Kamin RA, Wilson GS (1980) Anal Chem 52:1198

    Article  CAS  Google Scholar 

  27. Wen F, Dong YH, Feng L, Wang S, Zhang SC, Zhang XR (2011) Anal Chem 83:1193

    Article  CAS  Google Scholar 

  28. Lavanya N, Radhakrishnan S, Sekar C (2012) Biosens Bioelectron 36:41

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the Fundamental Research Funds for the Central Universities (08143053).

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Authors and Affiliations

  1. School of Science, Xi’an Jiaotong University, Xi’an, China

    Wen Zhang, Cong Guo, Yingxing Chang, Fengyan Wu & Shujiang Ding

Authors
  1. Wen Zhang
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  2. Cong Guo
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  3. Yingxing Chang
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  4. Fengyan Wu
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  5. Shujiang Ding
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Correspondence to Wen Zhang.

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Zhang, W., Guo, C., Chang, Y. et al. Immobilization of horseradish peroxidase on zinc oxide nanorods grown directly on electrodes for hydrogen peroxide sensing. Monatsh Chem 145, 107–112 (2014). https://doi.org/10.1007/s00706-013-0979-6

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  • DOI: https://doi.org/10.1007/s00706-013-0979-6

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