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Ultrasensitive chemiluminescence detection of DNA on a microfluidic paper-based analytical device

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Abstract

A novel microfluidic paper-based chemiluminescence (CL) analytical device (μPAD) with high-throughput, rapid, stable, reusable point-of-care (POC) testing and quantitative response for DNA was designed. Sodium periodate was used to modify μPAD to covalently immobilize capture DNA. The paper device was fabricated by a low-cost, simple, and rapid wax screen-printing method. The developed paper-based CL immunodevice, combined with a typical luminol–H2O2 CL system, showed excellent analytical performance for the detection of DNA. Moreover, gold nanoparticles for signal DNA immobilization were used for signal-on CL assay of target DNA, which greatly improved the sensitivity. This study shows the successful integration of the μPAD and the CL method to afford an easy-to-use, inexpensive, and portable alternative for POC monitoring.

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Acknowledgments

This work was financially supported by Natural Science Research Foundation of China (21277058, 21175058), Natural Science Foundation of Shandong Province, China (ZR2012BZ002), and Technology Development Plan of Shandong Province, China (2011GGB01153, 2011GGX10319).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Yanhu Wang, Shoumei Wang, Shenguang Ge, Shaowei Wang, Mei Yan, Dejin Zang & Jinghua Yu

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  1. Yanhu Wang
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  2. Shoumei Wang
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  3. Shenguang Ge
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  4. Shaowei Wang
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  5. Mei Yan
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  6. Dejin Zang
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  7. Jinghua Yu
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Correspondence to Jinghua Yu.

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Wang, Y., Wang, S., Ge, S. et al. Ultrasensitive chemiluminescence detection of DNA on a microfluidic paper-based analytical device. Monatsh Chem 145, 129–135 (2014). https://doi.org/10.1007/s00706-013-0971-1

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  • DOI: https://doi.org/10.1007/s00706-013-0971-1

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