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Determination of gabapentin in human plasma using simultaneous cloud point extraction and precolumn derivatization by HPLC

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Abstract

A simultaneous cloud point extraction and precolumn derivatization method was developed for the determination of gabapentin in human plasma samples. o-Phthaldialdehyde in the presence of 3-mercaptopropionic acid and Triton X-100 were used as derivatization reagents and extraction solvent, respectively. Some parameters with an effect on the extraction, such as the concentration of Triton X-100, incubation time, equilibration temperature, ionic strength, and centrifuging time, were studied and optimized. Under the optimum conditions, average recovery of gabapentin was in the range of 94.5–98.7 %. The calibration curve was linear in the range of 0.01–50 μg/cm3. The limits of detection and quantification were 0.003 and 0.01 μg/cm3, respectively. The relative standard deviations were in the range of 0.9–5.2 %. The obtained results show that simultaneous derivatization and cloud point extraction combined with high-performance liquid chromatography is a sensitive and simple method for the determination of gabapentin in plasma samples.

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References

  1. Ouellet D, Bockbrader HN, Wesche DL, Shapiro DY, Garofalo E (2001) Epilepsy Res 47:229

    Article  CAS  Google Scholar 

  2. Gidal BE, Radulovic LL, Kruger S, Rutecki P, Pitterle M, Bockbrader HN (2000) Epilepsy Res 40:123

    Article  CAS  Google Scholar 

  3. Hooper WD, Kavanagh MC, Dickinson RG (1990) J Chromatogr 529:167

    Article  CAS  Google Scholar 

  4. Wolf CE, Saady JI, Poklis A (1996) J Anal Toxicol 20:498

    Article  CAS  Google Scholar 

  5. Kushnir MM, Crossett J, Brown PI, Urry FM (1999) J Anal Toxicol 23:1

    Article  CAS  Google Scholar 

  6. Borrey DCR, Godderis KO, Engelrelst VIL, Bernard DR, Langlois MR (2005) Clin Chim Acta 354:147

    Article  CAS  Google Scholar 

  7. Lensmeyer GL, Kempf T, Gidal BE, Wiebe DA (1995) Ther Drug Monit 17:247

    Article  Google Scholar 

  8. Hengy H, Kölle E (1985) J Chromatogr 341:473

    Article  CAS  Google Scholar 

  9. Zhu Z, Neirinck L (2002) J Chromatogr B 779:307

    Article  CAS  Google Scholar 

  10. Ifa DR, Falci M, Moraes ME, Bezerra FAF, Moraes MO, de Nucci G (2001) J Mass Spectrom 36:188

    Article  CAS  Google Scholar 

  11. Ji HY, Jeong DW, Kim YH, Kim HH, Yoon YS, Lee KC, Lee HS (2006) Rapid Commun Mass Spectrom 20:2127

    Article  CAS  Google Scholar 

  12. Garcia LL, Shihabi ZK, Oles K (1995) J Chromatogr B 669:157

    Article  CAS  Google Scholar 

  13. Rada P, Tucci S, Perez J, Teneud L, Chuecos S, Hernandez L (1998) Electrophoresis 19:2976

    Article  CAS  Google Scholar 

  14. Chang SY, Wang FY (2004) J Chromatogr B 799:265

    Article  CAS  Google Scholar 

  15. Jalalizadeh H, Souri E, Barazandeh Tehrani M, Jahangiri A (2007) J Chromatogr B 854:43

    Article  CAS  Google Scholar 

  16. Wad N, Krämer G (1998) J Chromatogr B 705:154

    Article  CAS  Google Scholar 

  17. Gauthier D, Gupta R (2002) Clin Chem 12:2259

    Google Scholar 

  18. Chollet DF, Goumaz L, Juliano C, Anderegg G (2000) J Chromatogr B 746:311

    Article  CAS  Google Scholar 

  19. Tang PH, Miles MV, Glauser TA, DeGrauw T (1999) J Chromatogr B 727:125

    Article  CAS  Google Scholar 

  20. Juergens UH, May TW, Rambeck B (1996) J Liq Chrom Relat Technol 19:1459

    Article  CAS  Google Scholar 

  21. Ratnaraj N, Patsalos NP (1998) Ther Drug Monit 20:430

    Article  CAS  Google Scholar 

  22. Forrest G, Sills GJ, Leach JP, Brodie MJ (1996) J Chromatogr B 681:421

    Article  CAS  Google Scholar 

  23. Jiang Q, Li S (1999) J Chromatogr B 727:119

    Article  CAS  Google Scholar 

  24. Lindroth P, Mopper K (1979) Anal Chem 51:1667

    Article  CAS  Google Scholar 

  25. Hodgin JC (1979) J Liq Chromatogr 2:1047

    Article  CAS  Google Scholar 

  26. Roth M (1971) Anal Chem 43:880

    Article  CAS  Google Scholar 

  27. Pramauro E, Prevot AB (1995) Pure Appl Chem 67:551

    Article  CAS  Google Scholar 

  28. Saitoh T, Hinze WL (1991) Anal Chem 63:2520

    Article  CAS  Google Scholar 

  29. Hinze WL, Singh HN, Baba Y, Harvey NG (1984) TrAC Trends Anal Chem 3:193

    Article  CAS  Google Scholar 

  30. Fröschl B, Stangl G, Niessner R (1997) Fresenius J Anal Chem 357:743

    Article  Google Scholar 

  31. Okada T (1992) Anal Chem 64:2138

    Article  CAS  Google Scholar 

  32. Vermeij TAC, Edelbroek PM (2004) J Chromatogr B 810:297

    CAS  Google Scholar 

  33. Siddiqui FA, Arayne MS, Sultana N, Qureshi F, Mirza AZ, Zuberi MH, Bahadur SS, Afridi NS, Shamshad H, Rehman N (2010) Eur J Med Chem 45:2761

    Article  CAS  Google Scholar 

  34. Belal F, Abdine H, Al-Majed A, Khalil NY (2002) J Pharm Biomed Anal 27:253

    Article  CAS  Google Scholar 

  35. Manera M, Mirό M, Ribeiro MFT, Estela JM, Cerdà V, Santos JLM, Lima JLFC (2009) Luminescence 24:10

    Article  CAS  Google Scholar 

  36. Matar KM, Abdel-Hamid ME (2005) Chromatographia 61:499

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the support of the Islamic Azad University of Khorramabad and Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences. The authors thank Dr. B. Rasoulian for his excellent collaboration.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran

    Zeinab Pourghobadi, Rouhollah Heydari & Roghiyeh Pourghobadi

  2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

    Marzieh Rashidipour

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  1. Zeinab Pourghobadi
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  2. Rouhollah Heydari
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  3. Roghiyeh Pourghobadi
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  4. Marzieh Rashidipour
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Correspondence to Rouhollah Heydari.

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Pourghobadi, Z., Heydari, R., Pourghobadi, R. et al. Determination of gabapentin in human plasma using simultaneous cloud point extraction and precolumn derivatization by HPLC. Monatsh Chem 144, 773–779 (2013). https://doi.org/10.1007/s00706-012-0879-1

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  • DOI: https://doi.org/10.1007/s00706-012-0879-1

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