Summary.
In order to clarify the biological role of US2 gene product of herpes simplex virus type 2 (HSV-2), a HeLa cDNA library was screened in the yeast two-hybrid system using US2 protein as bait, and several interacting proteins were identified, including cytokeratin 18. US2 protein was co-immunoprecipitated with cytokeratin 18 from HSV-2 infected cell lysates. Analysis of infected or A431 cells by immunofluorescence showed that US2 protein gave filamentous or dot-like cytoplasmic staining pattern, and that it co-localized with cytokeratin 18. When US2 protein was expressed alone, it co-localized with cytokeratin 18. To define the domain interacting with cytokeratin 18, deletion mutant proteins were constructed and cells transfected with mutants were analyzed by indirect immuno-fluorescence. These results suggest that the N-terminal half of the US2 protein, especially the region containing amino acids 42–77, is important for interaction with cytokeratin 18.
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Received June 4, 2001 Accepted June 25, 2001
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Goshima, F., Watanabe, D., Suzuki, H. et al. The US2 gene product of herpes simplex virus type 2 interacts with cytokeratin 18. Arch. Virol. 146, 2201–2209 (2001). https://doi.org/10.1007/s007050170030
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DOI: https://doi.org/10.1007/s007050170030