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Molecular characteristics of a novel hypovirus from Trichoderma harzianum

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Abstract

We report a novel mycovirus with a positive-sense single-stranded (+)ss RNA genome, belonging to the family Hypoviridae, infecting Trichoderma harzianum strain M6. The complete genome sequence is 13,813 nucleotides long, excluding the poly(A) tail at the 3′ end. Sequence analysis revealed that the genome has a single large open reading frame (ORF) encoding a 4,118-amino-acid polyprotein harboring five conserved motifs of a protease, two conserved domains of a protein of unknown function, an RNA-dependent RNA polymerase, and a helicase. Sequence comparisons revealed that the deduced amino acid sequence of the polyprotein is similar to those of other hypoviruses and is most similar to that of Bipolaris oryzae hypovirus 1 (35.1% identity). Phylogenetic analysis using full-length RdRp and helicase sequences showed that this virus clustered closely with known members of the proposed genus “Alphahypovirus” of the family Hypoviridae. We accordingly designated this novel mycovirus “Trichoderma harzianum hypovirus 2” (ThHV2).

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The genome sequence of the hypovirus described here has been submitted to the GenBank database.

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Acknowledgements

We wish to thank the Institute of Molecular Biology and Genetics at Jeonbuk National University for kindly providing the facilities for this research.

Funding

This work was supported by the NRF grants NRF-2018R1A2A1A05078682 and NRF-2019R1I1A1A01061618, and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through the Agri-Bio Industry Technology Development Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (317025-05-5-HD020). This research was supported by “Research Base Construction Fund Support Program” funded by Jeonbuk National University in 2021.

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  1. Jeesun Chun and Kum-Kang So contributed equally.

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  1. Department of Molecular Biology, Institute for Molecular Biology and Genetics, Jeonbuk National University, 567 Baekje-daero, Jeonju, 54896, Korea

    Jeesun Chun, Kum-Kang So, Yo-Han Ko & Dae-Hyuk Kim

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Dae-Hyuk Kim wrote the manuscript. Jeesun Chun and Kum-Kang So performed the experiments. Dae-Hyuk Kim, Jeesun Chun, Kum-Kang So, and Yo-Han Ko analyzed the data.

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Correspondence to Dae-Hyuk Kim.

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Chun, J., So, KK., Ko, YH. et al. Molecular characteristics of a novel hypovirus from Trichoderma harzianum. Arch Virol 167, 233–238 (2022). https://doi.org/10.1007/s00705-021-05253-1

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