Abstract
High-throughput sequencing (HTS) was used to construct the virome profile of an old grapevine-leafroll-diseased grapevine (Vitis vinifera). De novo assembly of HTS data showed a complex infection, including a virus sequence with similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 7090 nucleotides and has four open reading frames (ORFs). Genome organisation and phylogenetic analysis identify this virus as a divergent variant of grapevine Roditis leaf discoloration-associated virus (GRLDaV) with 90% nucleotide sequence identity to isolate w4 (NC_027131). This is the first genome sequence of a South African variant of GRLDaV.
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This study was partially funded by VirFree. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant agreement no. 734736. This publication reflects only the authors’ view. The Agency is not responsible for any use that may be made of the information it contains. The financial assistance of Winetech (P04000069) towards this research is hereby acknowledged.
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Bester, R., Lotos, L., Vermeulen, A. et al. Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa. Arch Virol 166, 2041–2044 (2021). https://doi.org/10.1007/s00705-021-05079-x
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DOI: https://doi.org/10.1007/s00705-021-05079-x