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Partial biological and molecular characterization of a novel citrivirus from Nandina domestica

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Abstract

We report the complete genome sequence of a novel virus isolated from Nandina domestica ‘Firepower’ in Auckland, New Zealand. It was mechanically transmitted to Nicotiana species, although all of these infections were symptomless. The complete genome of the new virus is 8892 nucleotides (nt) long, excluding the 3′ poly(A) tail, contains three open reading frames (ORF), and is most closely related to citrus leaf blotch virus (CLBV) Actinidia isolate (CLBV-Act; 72% nt sequence identity), a member of the genus Citrivirus. Replicase and coat proteins, encoded by genome ORFs 1 and 3 respectively, shared 81–83% and 76–79% amino acid (aa) sequence identity, respectively, with CLBV-Act. Computer-based analysis suggests that this novel virus is the result of recombination between CLBV-Act and an unknown virus, highlighting the importance of this phenomenon for betaflexivirus evolution.

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Acknowledgements

We would like to thank Emma Scheltema from the Ministry for Primary Industries (MPI) for editing the figures, and Luciano Rigano from MPI for reviewing the manuscript.

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Authors and Affiliations

  1. Plant Health and Environment Laboratory, Ministry for Primary Industries, P.O. Box 2095, Auckland, 1140, New Zealand

    Stella Veerakone, Lia W. Liefting, Subuhi Khan, Chandan Pal & Joe Tang

  2. Royal Horticultural Society (RHS), London, UK

    Lisa I. Ward

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  1. Stella Veerakone
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  2. Lia W. Liefting
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  3. Subuhi Khan
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  4. Chandan Pal
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  5. Joe Tang
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  6. Lisa I. Ward
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Correspondence to Stella Veerakone.

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Veerakone, S., Liefting, L.W., Khan, S. et al. Partial biological and molecular characterization of a novel citrivirus from Nandina domestica. Arch Virol 166, 1395–1399 (2021). https://doi.org/10.1007/s00705-020-04868-0

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  • DOI: https://doi.org/10.1007/s00705-020-04868-0

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