Abstract
In this study, the complete genomic sequence of a novel botoulivirus (Sclerotinia minor botoulivirus 1, SmBV1) from the phytopathogenic fungus Sclerotinia minor strain LC45 was determined. The genome of SmBV1 is 2,882 nucleotides in length and contains a single large open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis showed that SmBV1 clustered with the botoulivirus clade within the family Botourmiaviridae. This is the first report of a botoulivirus in S. minor.
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References
Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384
Xie JT, Jiang DH (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68
Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368
Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128
Anagnostakis SL (1982) Biological control of chestnut blight. Science 215:466–471
Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe. Annu Rev Phytopathol 32:581–599
Yu X, Li B, Fu YP, Jiang DH, Ghabrial SA, Li GQ, Peng YL, Xie JT, Cheng JS, Huang JB, Yi XH (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392
Yu X, Li B, Fu YP, Xie JT, Cheng JS, Ghabrial SA, Li GQ, Yi XH, Jiang DH (2013) Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc Natl Acad Sci USA 110:1452–1457
Donaire L, Rozas J, Ayllón MA (2016) Molecular characterization of Botrytis ourmia-like virus, a mycovirus close to the plant pathogenic genus Ourmiavirus. Virology 489:158–164
Illana A, Marconi M, Rodríguez-Romero J, Xu P, Dalmay T, Wilkinson MD, Ayllón MÁ, Sesma A (2017) Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae. Arch Virol 162:891–895
Ohkita S, Lee Y, Nguyen Q, Ikeda K, Suzuki N, Nakayashiki H (2019) Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae. Virology 534:25–35
Guo J, Zhu JZ, Zhou XY, Zhong J, Li CH, Zhang ZG, Zhu HJ (2019) A novel ourmia-like mycovirus isolated from the plant pathogenic fungus Colletotrichum gloeosporioides. Arch Virol 164:2631–2635
Li CX, Zhu JZ, Gao BD, Zhu HJ, Zhou Q, Zhong J (2019) Characterization of a novel ourmia-like mycovirus infecting Magnaporthe oryzae and implications for viral diversity and evolution. Viruses 11:223
Abawi GS, Grogan RG (1979) Epidemiology of diseases caused by Sclerotinia species. Phytopathology 69:899–904
Melzer MS, Smith EA, Boland GJ (1997) Index of plant hosts of Sclerotinia minor. Can J Plant Pathol 19:272–280
Gaetan SA, Madia M (2008) Occurrence of Sclerotinia stem rot on canola caused by Sclerotinia minor in Argentina. Plant Dis 92:172
Lyu A, Zhang J, Yang L, Li GQ (2015) First report of Sclerotinia minor on Brassica rapa subsp. pekinensis in central China. Plant Dis 98:992
Yang D, Wu M, Zhang J, Chen W, Li G, Yang L (2018) Sclerotinia minor endornavirus 1, a novel pathogenicity debilitation-associated mycovirus with a wide spectrum of horizontal transmissibility. Viruses 10:589
Melzer MS, Boland GJ (1996) Transmissible hypovirulence in Sclerotinia minor. Can J Plant Pathol 18:19–28
Yang D, Zhang J, Wu MD, Chen WD, Li GQ, Yang L (2016) Characterization of the mycelial compatibility groups and mating type alleles in populations of Sclerotinia minor in central China. Plant Dis 100:2313–2318
Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
Yu L, Sang W, Wu MD, Zhang J, Yang L, Zhou YJ, Chen WD, Li GQ (2015) Novel hypovirulence-associated RNA mycovirus in the plant pathogenic fungus Botrytis cinerea. Appl Environ Microbiol 81:2299–2310
Wu MD, Zhang L, Li GQ, Jiang DH, Hou MS, Huang HC (2007) Hypovirulence and double-stranded RNA in Botrytis cinerea. Phytopathology 97:1590–1599
Wu M, Zhang L, Li G, Jiang D, Ghabrial SA (2010) Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea. Virology 406:117–126
Wu MD, Jin FY, Zhang J, Yang L, Jiang DH, Li GQ (2012) Characterization of a novel bipartite double-stranded RNA mycovirus conferring hypovirulence in the phytopathogenic fungus Botrytis porri. J Virol 86:6605–6619
Jiang DH, Ghabrial SA (2004) Molecular characterization of Penicillium chrysogenum virus: reconsideration of the taxonomy of the genus Chrysovirus. J Gen Virol 85:2111–2121
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This research was financially supported by the National Natural Science Foundation of China (NSFC) (grant no. 31701832) and the National Key Research and Development program of China (2017YFD0201100).
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Liang, N., Yang, D., Wu, M. et al. Molecular characterization of a novel botoulivirus from the phytopathogenic fungus Sclerotinia minor. Arch Virol 165, 785–788 (2020). https://doi.org/10.1007/s00705-020-04530-9
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DOI: https://doi.org/10.1007/s00705-020-04530-9