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First detection and genetic characterization of a novel kirkovirus from a dead thoroughbred mare in northern Xinjiang, China, in 2018

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Abstract

Background

In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cause of horse death, using virome.

Results

We have identified an Equ1-like virus from the fecal virome of a dead thoroughbred mare in China. Full genomic sequencing and phylogenetic analysis of the virus, tentatively named “kirkovirus Cj-7-7”, showed that it was closely related to kirkovirus Equ1 and clustered together with po-circo-like viruses 21, 22, 41, and 51, suggesting that it should be assigned to the proposed family “Kirkoviridae”. An epidemiological investigation showed that kirkovirus Cj-7-7 circulates in horses of northern Xinjiang and may specifically infect intestinal cells.

Conclusions

Our findings demonstrate the genetic diversity and geographic distribution of Kirkoviruses, and the prevalence of Kirkovirus Cj-7-7 in Xinjiang, China.

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Acknowledgements

This study was supported by the Natural Science Foundation of Xinjiang Uyghur Autonomous Region (2019D01A47), China Postdoctoral Science Foundation (2019M653901XB), and Prior Period Project of Xinjiang Agricultural University (XJAU201721). We thank the Postdoctoral Science Foundation of Xinjiang Agricultural University and Xinjiang Uygur Autonomous Region High-Level Talent Introduction.

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Author notes

  1. Jinxin Xie, Panpan Tong, and Aoyuntuya Zhang contributed equally.

Authors and Affiliations

  1. College of Veterinary Medicine, Xinjiang Agricultural University, Wulumuqi, 830052, Xinjiang Province, People’s Republic of China

    Jinxin Xie, Panpan Tong, Aoyuntuya Zhang, Yongtao Yan, Lei Zhang, Xiaozhen Song, Jianxing Chen, Shaohua Zhai, Dechao Wang, Zhanqiang Su & Ling Kuang

  2. Yili Center of Animal Disease Control and Diagnosis, Yili Animal Health Inspection, Yining, 835000, Xinjiang Province, People’s Republic of China

    Nuerlan Shaya

Authors
  1. Jinxin Xie
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  2. Panpan Tong
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  3. Aoyuntuya Zhang
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  4. Yongtao Yan
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  5. Lei Zhang
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  6. Xiaozhen Song
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  7. Jianxing Chen
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  8. Shaohua Zhai
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  9. Nuerlan Shaya
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  10. Dechao Wang
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  11. Zhanqiang Su
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  12. Ling Kuang
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Corresponding authors

Correspondence to Zhanqiang Su or Ling Kuang.

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Conflict of interest

The authors have declared no competing interests.

Ethics statement

All experimental procedures involving animals were approved by the Animal Care and Use Committee of Xinjiang Agricultural University, Wulumuqi, Xinjiang, China (animal protocol number 2018005).

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Handling Editor: William G Dundon.

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Xie, J., Tong, P., Zhang, A. et al. First detection and genetic characterization of a novel kirkovirus from a dead thoroughbred mare in northern Xinjiang, China, in 2018. Arch Virol 165, 403–406 (2020). https://doi.org/10.1007/s00705-019-04487-4

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  • DOI: https://doi.org/10.1007/s00705-019-04487-4

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