Abstract
Here, we describe the complete genome sequence of melon yellowing-associated virus (MYaV), found in melon plants with severe yellowing disease, determined by high-throughput and Sanger sequencing. MYaV has an RNA genome of 9073 nucleotides plus a poly(A) tail. At least six open reading frames were predicted, with a typical carlavirus genomic organisation. Phylogenetic analysis of the complete genome sequence and the amino acid sequences of the RNA-dependent RNA polymerase confirmed that MYaV belongs to the genus Carlavirus, with the highest genome-wide nucleotide sequence identity of 59.8% to sweet potato yellow mottle virus.
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Acknowledgements
This work was supported by CAPES/Brazil for TMC and the program Ciência sem Fronteiras (CNPq/Brazil) for MAA.
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705_2017_3532_MOESM1_ESM.pptx
Supplementary Fig. 1: Schematic representation of the genome organisation and amplification by RT-PCR. A. Organization, identification, and size of the ORFs in the MYaV-M22 genome of 9037 nucleotides. 2, TGB2; 3, TGB3; NaBp, nucleic acid binding protein; Met, methyltransferase; Ox, 2OG-Fe(II) oxygenase; P, peptidase_C23; Hel, helicase and RdRp, RNA-dependent RNA polymerase. B. Position of the primers in the genome used for cDNA amplification by RT-PCR covering the genome for Sanger sequencing (PPTX 33 kb)
705_2017_3532_MOESM2_ESM.pptx
Supplementary Fig. 2. Matrix generated by Sequence Demarcation Tool (SDT) Version 1.2 comparing the amino acid sequences of RdRp of MYaV and related carlavirus species (PPTX 86 kb)
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Costa, T.M., Blawid, R., da Costa Junior, A.C. et al. Complete genome sequence of melon yellowing-associated virus from melon plants with the severe yellowing disease in Brazil. Arch Virol 162, 3899–3901 (2017). https://doi.org/10.1007/s00705-017-3532-9
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DOI: https://doi.org/10.1007/s00705-017-3532-9