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The biological features and genetic diversity of novel fish rhabdovirus isolates in China

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Abstract

The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 novel viruses from diseased fish collected from China including SCRV-QY, SCRV-SS, SCRV-GM, CmRV-FS, MsRV-SS, OmbRV-JM. The typical clinical symptom of diseased fish was hemorrhaging. Efficient propagation of these isolates in a Chinese perch brain cell line was determined by means of observation of cytopathic effect, RT-PCR and electron microscopy. Sequence alignment and phylogenetic analysis of the complete G protein sequences revealed that these isolates were clustered into one monophyletic lineage belonging to the species Siniperca chuatsi rhabdovirus.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Shanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100, Shanxi, China

    Xiaozhe Fu & Jianguo Su

  2. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, 510380, Guangdong, China

    Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Zhibin Huang & Ningqiu Li

Authors
  1. Xiaozhe Fu
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  2. Qiang Lin
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  3. Hongru Liang
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  4. Lihui Liu
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  5. Zhibin Huang
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  6. Ningqiu Li
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  7. Jianguo Su
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Corresponding authors

Correspondence to Ningqiu Li or Jianguo Su.

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Funding

This study was financially supported by the National Natural Science Fund (No. 31502201) and special funds for the Marine Fishery Science & Technology Promotion Project of Guangdong Provinces (A201501B12).

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Fu, X., Lin, Q., Liang, H. et al. The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Arch Virol 162, 2829–2834 (2017). https://doi.org/10.1007/s00705-017-3416-z

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  • DOI: https://doi.org/10.1007/s00705-017-3416-z

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