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Virus surveys of Capsicum spp. in the Republic of Benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species

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Abstract

Surveys were conducted in 2014 and 2015 in Southern and Northern Benin, respectively, to identify the viruses infecting peppers (Capsicum spp.). The samples were screened by ELISA for cucumber mosaic virus (CMV), pepper veinal mottle virus (PVMV), potato virus Y (PVY) and tomato yellow leaf curl virus (TYLCV). A generic reverse transcription PCR (RT-PCR) was used to test for the presence of poleroviruses. ELISA tests confirmed the prevalence of all viruses, while the RT-PCR detected pepper vein yellows virus (PeVYV) which is reported for the first time in Benin. A further, divergent polerovirus isolate was detected from a single pepper sample originating from southern Benin. Screening of samples collected from solanaceous plants during virus surveys in Mali (conducted in 2009) also detected this divergent polerovirus isolate in two samples from African eggplants. The complete genome sequence was obtained from the Mali isolate using transcriptome sequencing and by conventional Sanger sequencing of overlapping RT-PCR products. Based on the sequence characteristics of this isolate we propose a new polerovirus species, African eggplant yellowing virus (AeYV).

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Acknowledgements

The German Academic Exchange Service (DAAD) is acknowledged for providing reinitation scholarship to the 1st author.

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Authors and Affiliations

  1. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany

    Stephan Winter & Dennis Knierim

  2. The World Vegetable Center, PO Box 42, Shanhua, Tainan, 74199, Taiwan

    Lawrence Kenyon & Dennis Knierim

  3. Faculté d′Agronomie, Université de Parakou, 02 B.P., 1003, Parakou, Republic of Benin

    Leonard Afouda, Valerien Zinsou & Laurence Dossou

  4. University Felix Houphouet Boigny, Abidjan, Ivory Coast

    Daouda Kone

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  1. Leonard Afouda
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  2. Daouda Kone
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  3. Valerien Zinsou
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  4. Laurence Dossou
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  5. Lawrence Kenyon
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  6. Stephan Winter
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  7. Dennis Knierim
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Correspondence to Dennis Knierim.

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All authors (A. Leonard, D. Kone, V. Zinsou, L. Dossou, L. Kenyon, S. Winter, D. Knierim) declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Afouda, L., Kone, D., Zinsou, V. et al. Virus surveys of Capsicum spp. in the Republic of Benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species. Arch Virol 162, 1599–1607 (2017). https://doi.org/10.1007/s00705-017-3274-8

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  • DOI: https://doi.org/10.1007/s00705-017-3274-8

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