Abstract
Menghai rhabdovirus (MRV) was isolated from Aedes albopictus in Menghai county of Yunnan Province, China, in August 2010. Whole-genome sequencing of MRV was performed using an Ion PGM™ Sequencer. We found that MRV is a single-stranded, negative-sense RNA virus. The complete genome of MRV has 10,744 nt, with short inverted repeat termini, encoding five typical rhabdovirus proteins (N, P, M, G, and L) and an additional small hypothetical protein. Nucleotide BLAST analysis using the BLASTn method showed that the genome sequence most similar to that of MRV is that of Arboretum virus (NC_025393.1), with a Max score of 322, query coverage of 14%, and 66% identity. Genomic and phylogenetic analyses both demonstrated that MRV should be considered a member of a novel species of the family Rhabdoviridae.
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Acknowledgements
This research was supported by grants from the National Natural Science Foundation of China (No. 81273138 and No. 81572045), China Mega-Project on Infectious Disease Prevention (No. 2013ZX10004-605, No. 2013ZX10004-607, No. 2013ZX10004-217, and No. 2011ZX10004-001), and the National Hi-Tech Research and Development (863) Program of China (No. 2014AA021402, No. 2012AA022-003, and No. 2015AA020108).
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Q. Sun, Q. Zhao and X. An contributed equally to this work.
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Sun, Q., Zhao, Q., An, X. et al. Complete genome sequence of Menghai rhabdovirus, a novel mosquito-borne rhabdovirus from China. Arch Virol 162, 1103–1106 (2017). https://doi.org/10.1007/s00705-016-3188-x
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DOI: https://doi.org/10.1007/s00705-016-3188-x