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Characterization of isolates of equine infectious anemia virus in Brazil

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Abstract

Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.

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Authors and Affiliations

  1. Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil

    Dellane Martins Tigre, Camila Fonseca Lopes Brandão, Fabiana Lopes de Paula, Gubio Soares Campos & Silvia Ines Sardi

  2. Department of Biointeraction, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil

    Fabio Alexandre Chinalia & Silvia Ines Sardi

  3. Department of Biologic Science, Universidade Estadual do Sudoeste da Bahia (UESB), Jequie, Brazil

    Dellane Martins Tigre

  4. Instituto Federal Baiano (IFBA), Santa Inês, Brazil

    Camila Fonseca Lopes Brandão

  5. Institute of Health Science, Universidade Federal do Recôncavo da Bahia (UFRB), Santo Antonio de Jesus, Brazil

    Fabiana Lopes de Paula

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  1. Dellane Martins Tigre
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  2. Camila Fonseca Lopes Brandão
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  3. Fabiana Lopes de Paula
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  4. Fabio Alexandre Chinalia
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  5. Gubio Soares Campos
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Correspondence to Dellane Martins Tigre.

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The authors declare that they have no conflict of interest.

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Not required by this journal: study based on samples collected from equids subjected to euthanasia due to their disease status.

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Tigre, D.M., Brandão, C.F.L., de Paula, F.L. et al. Characterization of isolates of equine infectious anemia virus in Brazil. Arch Virol 162, 873–877 (2017). https://doi.org/10.1007/s00705-016-3172-5

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  • DOI: https://doi.org/10.1007/s00705-016-3172-5

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