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Molecular characterization of a new human coxsackievirus B2 associated with severe hand-foot-mouth disease in Yunnan Province of China in 2012

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Abstract

Human coxsackievirus B2 (CVB2) belongs to the species Human enterovirus B and can cause aseptic meningitis, myocarditis and hand-foot-mouth disease (HFMD). We first determined the complete genome of the RW41-2/YN/CHN/2012 strain, isolated from a patient with HFMD and aseptic meningitis in the Yunnan Province, China in 2012. The strain shared 83.5 % and 82.2 % nucleotide similarity with CVB2 prototype strain Ohio-1, in the complete VP1 gene and the complete genome, respectively. Using phylogenetic and homogeneity analyses for the complete VP1 gene, CVB2 strains could be divided into four genogroups (A–D); the RW41-2/YN/CHN/2012 strain belonging to genogroup D. The amino acid sequence of VP1 is highly conserved. Recombination analyses showed the newly isolated RW41-2/YN/CHN/2012 strain was probably a recombinant, which was closely related to strain CVB2 (KM386639) in the genomic P1 and P2 regions and strains of other human enterovirus B (HEV-B) viruses (KT353721, JX644073, and KP262053) in the P3 region.

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Acknowledgements

This work was supported by the Basic Research Projects of Yunnan Province, China (Grant Number: 2013FZ136)

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    Authors and Affiliations

    1. Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China

      Jie Zhang, Haihao Zhang, Yilin Zhao, Chen Guo, Zhaoqing Yang & Shaohui Ma

    2. Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China

      Jie Zhang, Haihao Zhang, Yilin Zhao, Chen Guo, Zhaoqing Yang & Shaohui Ma

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    1. Jie Zhang
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    2. Haihao Zhang
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    3. Yilin Zhao
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    4. Chen Guo
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    5. Zhaoqing Yang
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    6. Shaohui Ma
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    Correspondence to Zhaoqing Yang or Shaohui Ma.

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    All experimental protocols involving human samples were approved by the Human Ethics Commission at the Institute of Medical Biology, Chinese Academy of Medical Sciences.

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    J. Zhang and H. Zhang have contributed equally to this study.

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    Zhang, J., Zhang, H., Zhao, Y. et al. Molecular characterization of a new human coxsackievirus B2 associated with severe hand-foot-mouth disease in Yunnan Province of China in 2012. Arch Virol 162, 307–311 (2017). https://doi.org/10.1007/s00705-016-3075-5

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    • DOI: https://doi.org/10.1007/s00705-016-3075-5

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