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Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.)

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Abstract

A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RT-PCR with specific primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).

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Author contribution statement

LL and LC detected the disease, carried out field monitoring, symptoms observation and samples collection. PS, MM and AG conceived and designed the lab experiments. AG performed RNA extraction, library preparation and NGS sequencing. AG and MM performed NGS data analysis. MM performed NGS experimental validation and conventional sequencing. MM performed genomic characterization and phylogenetic analysis. MM designed and performed the RT-PCR assay. PS and VNS contributed to data interpretation. MM and PS draft the paper. VNS critically reviewed the manuscript. All authors read and approved the manuscript.

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Authors and Affiliations

  1. CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), Via Amendola 122/D, 70126, Bari, Italy

    Massimiliano Morelli, Annalisa Giampetruzzi & Pasquale Saldarelli

  2. Agrimeca Grape and Fruit Consulting S.r.l., Via Elefante 17, 70010, Turi, Bari, Italy

    Lorenzo Laghezza & Luigi Catalano

  3. Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi Aldo Moro, Bari, Italy

    Vito Nicola Savino

Authors
  1. Massimiliano Morelli
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  2. Annalisa Giampetruzzi
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  3. Lorenzo Laghezza
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  4. Luigi Catalano
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  5. Vito Nicola Savino
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  6. Pasquale Saldarelli
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Correspondence to Massimiliano Morelli.

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MM declares that he has no conflict of interest. AG declares that she has no conflict of interest. LL declares that he has no conflict of interest. LC declares that he has no conflict of interest. VNS declares that he has no conflict of interest. PS declares that he has no conflict of interest.

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This article does not contain any study with human participants or animals performed by any of the authors.

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Morelli, M., Giampetruzzi, A., Laghezza, L. et al. Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.). Arch Virol 162, 299–306 (2017). https://doi.org/10.1007/s00705-016-3074-6

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  • DOI: https://doi.org/10.1007/s00705-016-3074-6

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