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Apigenin inhibits African swine fever virus infection in vitro

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Abstract

African swine fever virus (ASFV) is one of the most devastating diseases of domestic pigs for which no effective vaccines are available. Flavonoids, natural products isolated from plants, have been reported to have significant in vitro and in vivo antiviral activity against different viruses. Here, we tested the antiviral effect of five flavonoids on the replication of ASFV in Vero cells. Our results showed a potent, dose-dependent anti-ASFV effect of apigenin in vitro. Time-of-addition experiments revealed that apigenin was highly effective at the early stages of infection. Apigenin reduced the ASFV yield by more than 99.99 % when it was added at 1 hpi. The antiviral activity of apigenin was further investigated by evaluation of ASFV protein synthesis and viral factories. This flavonoid inhibited ASFV-specific protein synthesis and viral factory formation. ASFV-infected cells continuously treated with apigenin did not display a cytopathic effect. Further studies addressing the use of apigenin in vivo are needed.

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Acknowledgements

We thank ARMACAD for investments in laboratory renovation. We thank Dr. Haig Eskandarian (EPFL, Switzerland) for critical reading and useful comments.

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Authors and Affiliations

  1. Group of Antiviral Defense Mechanisms, Institute of Molecular Biology of NAS RA, 0014, Yerevan, Armenia

    Astghik Hakobyan, Erik Arabyan & Hovakim Zakaryan

  2. Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 0014, Yerevan, Armenia

    Aida Avetisyan, Liana Abroyan & Lina Hakobyan

  3. Yerevan State Medical University after M. Heratsi, 0025, Yerevan, Armenia

    Hovakim Zakaryan

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  1. Astghik Hakobyan
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Correspondence to Hovakim Zakaryan.

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This work was supported by a Grant from the Armenian National Science and Education Fund (Biotech-3751). All authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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Hakobyan, A., Arabyan, E., Avetisyan, A. et al. Apigenin inhibits African swine fever virus infection in vitro . Arch Virol 161, 3445–3453 (2016). https://doi.org/10.1007/s00705-016-3061-y

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  • DOI: https://doi.org/10.1007/s00705-016-3061-y

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