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Interleukin-10.rs1800896 and Interleukin-18.rs1946518 gene polymorphisms could not predict the outcome of hepatitis C virus infection in Egyptian patients treated with pegylated interferon plus ribavirin

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Abstract

A single-nucleotide polymorphism (SNP) in the interleukin (IL)-28B gene was used as a major predictor of the response to treatment in patients with hepatitis C virus (HCV) infection. Data examining the role of IL-10 and IL-18 gene polymorphisms among HCV genotype 4 (G4)-infected Egyptians in response to pegylated interferon (PEG-IFN) plus ribavirin (RBV) therapy are limited. This study investigated the impact of SNP at IL-10.rs1800896 (at position -1082) and IL-18.rs1946518 genes (at position -607) on the response to PEG-IFN/RBV therapy in HCV-infected Egyptians. This study was carried out on 100 HCV patients treated with PEG-IFN plus RBV and 100 healthy controls. The HCV patients included 50 treatment non-responders (NR) and 50 subjects with sustained virologic response (SVR). Genomic DNA from venous blood of subjects was extracted and IL-10.rs1800896 and IL-18.rs1946518 genotypes were determined using allele-specific amplification and SYBR Green real-time PCR. Linkage disequilibrium between the two SNPs was estimated using Haploview software. The frequency of the IL-10.rs1800896 AA, AG and GG genotypes among non-responders were 16 %, 70 % and 14 % while among SVR subjects, the frequency was 34 %, 60 % and 6 %, respectively (p=0.073). On the other hand, the frequency of the IL-18.rs1946518 AA, AC and CC genotypes among non-responders was 14 %, 50 % and 36 %, respectively, while among responders, these frequencies were 28 %, 44 % and 28 %, (p = 0.220). Both markers were in linkage equilibrium (D’ = 0.23; r 2 = 0.052). SNPs in the IL-10.rs1800896 and IL-18.rs1946518 genes could not predict the outcome of HCV infection in Egyptians treated with PEG-IFN/RBV.

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Acknowledgments

We would like to thank Dr. Wesam Menisi and the physicians and nurses at the Commission for Hepatitis Viruses’ Treatment at Minia Health Insurance Hospital for their cooperation and sincere help in collecting the samples.

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Authors and Affiliations

  1. Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Wedad M. Abdelraheem, Noha A. Hassuna, Sahar M. Abuloyoun, Hazem A. Rizk & Sayed F Abdelwahab

  2. Biochemistry Department, Faculty of Medicine, Minia University, Minia, 61511, Egypt

    Hend M. Abdel Ghany

  3. Department of Microbiology, College of Pharmacy, Taif University, Al-Haweiah, PO Box 888, Taif, 21974, Kingdom of Saudi Arabia

    Sayed F Abdelwahab

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  1. Wedad M. Abdelraheem
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  5. Hazem A. Rizk
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Correspondence to Sayed F Abdelwahab.

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This work was supported by Minia University and personal funds.

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All authors declare that they have no conflict of interest.

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The research was carried out in accordance with the 1975 Helsinki Declaration. Also, the study protocol was ethically approved by the council of the Faculty of Medicine, Minia University.

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Abdelraheem, W.M., Hassuna, N.A., Abuloyoun, S.M. et al. Interleukin-10.rs1800896 and Interleukin-18.rs1946518 gene polymorphisms could not predict the outcome of hepatitis C virus infection in Egyptian patients treated with pegylated interferon plus ribavirin. Arch Virol 161, 2473–2480 (2016). https://doi.org/10.1007/s00705-016-2948-y

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