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Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

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Abstract

The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.

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Authors and Affiliations

  1. Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil

    Joana D. P. Mascarenhas, Delana A. M. Bezerra, Mayara J. M. Silva, Edivaldo C. Sousa Júnior & Luana S. Soares

  2. Laboratório Nacional Agropecuário (LANAGRO), Ministério da Agricultura, Belém, Brazil

    René R. Silva

Authors
  1. Joana D. P. Mascarenhas
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  2. Delana A. M. Bezerra
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  3. René R. Silva
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  4. Mayara J. M. Silva
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  5. Edivaldo C. Sousa Júnior
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  6. Luana S. Soares
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Corresponding author

Correspondence to Joana D. P. Mascarenhas.

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Funding

This study was partially supported by a grant from Pará State Secretary of Science and Technology, contract no. 067/2008 (SEDECT/FAPESPA/PA).

Conflict of interest

M.J.M.S and D.A.M.B. were supported by the National Council for Scientific and Technological Development (CNPq). J.D.P.M., R.R.S, E.C.S.J and L.S.S. declare that had no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This study was approved by the Ethics Committee on Animal Research of Instituto Evandro Chagas under number 0004/2007.

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Mascarenhas, J.D.P., Bezerra, D.A.M., Silva, R.R. et al. Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil. Arch Virol 161, 2263–2268 (2016). https://doi.org/10.1007/s00705-016-2882-z

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  • DOI: https://doi.org/10.1007/s00705-016-2882-z

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