Abstract
A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8–99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.
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Acknowledgments
We are grateful to Dr. Chihiro Yoshihara (RIKEN Brain Science Institute, Wako, Japan) and Mr. Akira Kokubun, Ms. Akiko Suganuma, Ms. Kazue Arakawa, and Ms. Kaoru Makabe (Equine Research Institute, Japan Racing Association) for providing technical assistance.
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Nucleotide sequence data is available in the GenBank/EMBL/DDBJ databases under the accession numbers LC079086, LC079087, LC079088, and LC079089.
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Nemoto, M., Bannai, H., Tsujimura, K. et al. Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan. Arch Virol 161, 1691–1695 (2016). https://doi.org/10.1007/s00705-016-2840-9
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DOI: https://doi.org/10.1007/s00705-016-2840-9