Abstract
The genetic diversity of eggplant mottled dwarf virus (EMDV), a member of the family Rhabdoviridae, was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. Sequences corresponding to the N, X, P, Y, M and G ORFs as well as the untranslated regions (UTRs) between ORFs were determined from all isolates. Low genetic diversity was found in almost all genomic regions studied except for the X ORF and the UTRs, which were more variable, while interestingly, an EMDV isolate from caper possessed a truncated G gene sequence. Furthermore, low d N /d S ratios, indicative of purifying selection, were calculated for all genes. Phylogenetic analysis showed that the EMDV isolates clustered in three distinct subgroups based on their geographical origin, with the exception of one subgroup that consisted of isolates from northern Greece and Cyprus. Overall, the level of genetic diversity of EMDV differed between seed- and asexually propagated plants in our collection, and this could be related to the mode of transmission.
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References
Adam G, Gaedigk K, Mundry K (1983) Alterations of a plant rhabdovirus during successive mechanical transfers. Zeitschrift fur Pflanzenkrankheit und Pflanzenschutz 90:28–35
Al-Musa AM, Lockhart BEL (1990) Occurrence of Eggplant mottled dwarf virus in Jordan. J Phytopathol 128:283–287
Ali A, Li H, Schneider WL, Sherman DJ, Gray S, Smith D, Roossinck MJ (2006) Analysis of genetic bottlenecks during horizontal transmission of Cucumber mosaic virus. J Virol 80:8345–8350
Alfaro-Fernandez A, Cordoba-Selles C, Tornos T, Cebrian MC, Font MI (2011) First report of Eggplant mottled dwarf virus in Pittosporum tobira in Spain. Plant Dis 95:75
Babaie G, Izadpanah K (2003) Vector transmission of Eggplant mottled dwarf virus in Iran. J Phytopathol 151:679–682
Babaie G, Kouhi Habibi M, Massah A, Dizadji A, Izadinejad L, Simon A (2015) Complete genome sequence and genome analysis of Eggplant mottled dwarf virus-Iranian Isolate. J Phytopathol 163:331–341
Callaghan B, Dietzgen RG (2005) Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus. Arch Virol 150:1661–1667
Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772–783
Della Giustina W, Javoy M, Bansept P, Morel E, Balasse H, Goussard N, Passard C (2000) Les cicadelles du genre Anaceratagallia vectrice du virus responsable de la maladie de la peau de crapaud du concombre. PHM-Revue Horticole 420:40–43
Dietzgen RG, Calisher CH, Kurath G, Kuzmin IV, Rodriguez LL, Stone DM, Tesh RB, Tordo N, Walker PJ, Wetzel T, Whitfield AE (2012) Rhabdoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego, pp 686–713
El Maataoui M, Lockhart BEL, Lesemann DE (1985) Biological, serological, and cytopathological properties of Tomato vein yellowing virus occurring in tomato in Morocco. Phytopathology 75:109–115
Ferriol I, Ferrer RM, Luis-Arteaga M, Guerri J, Moreno P, Rubio L (2014) Genetic variability and evolution of broad bean wilt virus 1: role of recombination, selection and gene flow. Arch Virol 159:779–784
Fu ZF (2005) Genetic comparison of the rhabdoviruses from animals and plants. Curr Top Microbiol 292:1–24
Garcia-Arenal F, Fraile A, Malpica JM (2001) Variability and genetic structure of plant virus populations. Annu Rev Phytopathol 39:157–186
Garcia-Arenal F, McDonald BA (2003) An analysis of the durability of resistance to plant viruses. Phytopathology 93:941–952
Garrett RG, McLean GD (1983) The epidemiology of some aphid-borne viruses in Australia. In: Plumb RT, Thresh JM (eds) Plant virus epidemiology. The spread and the control of insect-borne viruses. Blackwell Scientific Publications, Oxford, pp 199–209
Gouy M, Guindon S, Gascuel O (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224
Graves MV, Pogany J, Romero J (1996) Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants. Sem Virol 7:399–408
Ismail ID, Milner JJ (1988) Isolation of defective interfering particles of Sonchus yellow net virus from chronically infected plants. J Gen Virol 69:999–1006
Katis NI, Chatzivassiliou EK, Clay C, Avgelis I, Manoussopoulos I, Lecoq H (2000) Occurrence of Eggplant mottled dwarf nucleorhabdovirus (EMDV) in tobacco and cucumber crops in Greece. Phytopathol Mediterr 39:319
Katis NI, Chatzivassiliou EK, Clay CM, Maliogka VI, Pappi P, Efthimiou K, Dovas CI, Avgelis AD (2011) Development of an IC-RT-PCR assay for the detection of Eggplant mottled dwarf virus and partial characterization of isolates from various hosts in Greece. J Plant Pathol 93:353–362
Klerks MM, Lindner JL, Vaskova D, Spak J, Thompson JR (2004) Detection and tentative grouping of Strawberry crinkle virus isolates. Eur J Plant Pathol 110:45–52
Lai MM (1992) RNA recombination in animal and plant viruses. Microbiol Rev 56:61–79
Li H, Roossinck MJ (2004) Genetic bottlenecks reduce population variation in an experimental RNA virus population. J Virol 78:10582–10587
Maliogka VI, Olmos A, Pappi PG, Lotos L, Efthimiou K, Grammatikaki G, Candresse T, Katis NI, Avgelis AD (2015) A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease. Virus Res 203:47–55
Marco CF, Aranda MA (2005) Genetic diversity of a natural population of Cucurbit yellow stunting disorder virus. J Gen Virol 86:815–822
Martelli GP (1969) Bacilliform particles associated with mottled dwarf of eggplant (Solanum melongena L.). J Gen Virol 5:319–320
Martelli GP, Russo M, Rubino L (2011) Eggplant mottled dwarf virus. In: Description of plant viruses. Association of Applied Biologist, No. 42. http://www.dpvweb.net/dpv/showdpv.php?dpvno=421. Accessed Apr 2011
Martin S, Garcia ML, Troisi A, Rubio L, Legarreta G, Grau O, Alioto D, Moreno P, Guerri J (2006) Genetic variation of populations of Citrus psorosis virus. J Gen Virol 87:3097–3102
Moury B, Desbiez C, Jacquemond M, Lecoq H (2006) Genetic diversity of plant virus populations: towards hypothesis testing in molecular epidemiology. Adv Virus Res 67:49–87
Pappi PG, Dovas CI, Efthimiou KE, Maliogka VI, Katis NI (2013) A novel strategy for the determination of a rhabdovirus genome and its application to sequencing of Eggplant mottled dwarf virus. Virus Genes 47:105–113
Pappi PG, Efthimiou KE, Katis NI (2012) First report of Eggplant mottled dwarf virus in tobacco crops in Albania. J Plant Pathol 94:870
Parrella G, De Stradis A, Greco B, Villanueva F, Fortes IM, Navas-Castillo J (2013) First report of Eggplant mottled dwarf virus in China rose in southern Spain. Span J Agric Res 11:204–207
Revill P, Trinh X, Dale J, Harding R (2005) Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus. J Gen Virol 86:491–499
Roggero P, Milne RG, Masenga V, Ogliara P (1995) First report of Eggplant mottled dwarf rhabdovirus in cucumber and pepper. Plant Dis 79:321
Thekke-Veetil T, Polashock JJ, Marn MV, Plesko IM, Schilder AC, Keller KE, Martin RR, Tzanetakis IE (2015) Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates. Virus Res 201:79–84
Tsompana M, Abad J, Purugganan M, Moyer JW (2005) The molecular population genetics of the Tomato spotted wilt virus (TSWV) genome. Mol Ecol 14:53–66
Walker PJ, Dietzgen RG, Joubert DA, Blasdell KR (2011) Rhabdovirus accessory genes. Virus Res 162:110–125
Acknowledgements
The authors thank Prof. Benham E. L. Lockhart (Department of Plant Pathology, University of Minnesota, St. Paul, USA) and Dr. Lambros C. Papayiannis (Agricultural Research Institute, Nicosia, Cyprus) for kindly providing EMDV-specific antiserum and the EMDV-pt isolate, respectively.
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Fig. S1 Multiple nucleotide sequence alignment of a part of the G gene from the eight EMDV isolates, including the 275-nt deletion fragment of the EMDV-caps isolate from C. spinosa (PDF 341 kb)
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Fig. S2 Gel electrophoresis of RT-PCR products amplified using the primers G do 1398 and G up read (5’-GGACGATCCTGAATGCACTTACTG-3’). Lane 1, EMDV-caps isolate with a 275-nt deletion in the G gene; lanes 2, 4, 5, 6, 9, 10, and 12, isolates from infected C. spinosa tested with no deletion; lane 13, isolate from infected C. spinosa in which the two EMDV variants coexist; lanes 3, 7, 8, and 11, healthy capers; lane LD: 100 bp DNA ladder (PDF 21 kb)
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Fig. S3 Simplot analysis of the sequence spanning the 5’ end of the EMDV genome up to the 3’ end of the G ORF. The EMDV-pt isolate is used as a reference. The region corresponding to the 275-nt deletion was removed from all isolates before the analysis. The parameters used were a window size of 100 bp, a step size of 10 bp, and the Kimura 2-parameter model (PDF 42 kb)
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Fig. S4 Phylogenetic analysis of the region where potential recombination was detected (nt 862 to 1080 of the G [glycoprotein] gene). The tree was constructed by the BIONJ method, using the Jukes-Cantor model of evolution and 500 bootstrap replicates (PDF 14 kb)
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Pappi, P.G., Maliogka, V.I., Amoutzias, G.D. et al. Genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts. Arch Virol 161, 631–639 (2016). https://doi.org/10.1007/s00705-015-2705-7
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DOI: https://doi.org/10.1007/s00705-015-2705-7