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Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors

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Abstract

The human neurotropic JC virus (JCV) is of significant interest due to its experimental neuro- oncogenic potential. In clinical samples from human central nervous system (CNS) tumors, detection of JCV sequences suggests a possible association with CNS neoplasms, but the results are discrepant worldwide. To assess the prevalence of JCV sequences in Iranian patients with primary and metastatic CNS malignancies, a total of 58 fresh CNS tumors were examined by quantitative real-time PCR targeting the JCV large T antigen (LT-Ag) gene, and JCV DNA load was determined as viral copy number per cell. All patients were immunocompetent, and none of them had received immunosuppressive therapy before surgical operation. JC virus LT-Ag sequences were found in a total of 15 (25.9 %) out of the 58 tested samples. In primary CNS tumors, JCV sequences were identified more frequently in meningiomas (50.0 %) and schwannomas (35.7 %). In metastatic CNS tumors, JCV LT-Ag was identified in one case with brain adenocarcinoma originating from lung cancer. No statistically significant association between JCV positivity and various types of CNS malignancies was observed (P = 0.565). The mean JCV LT-Ag copy number in 15 positive cases was 1.8 × 10−4 ± 4.5 × 10−4 copies per cell (range 1.0 × 10−5-1.78 × 10−3 copies per cell). An inverse correlation between white blood cell (WBC) count and JCV copy number was observed, but this correlation was not statistically significant (R = −0.198, P = 0.480). This study provides the first data on the prevalence of JCV in primary and metastatic CNS tumors from Iranian patients.

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Acknowledgments

The authors would like to acknowledge the support of the directors and staff of the Neurosurgery Department of Shariati Hospital affiliated to Tehran University of Medical Sciences for their collaboration in sample collection. This study was financially supported by a grant from Tehran University of Medical Sciences (Project code: 92-01-30-20990).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Farzin Sadeghi & Mostafa Salehi-Vaziri

  2. Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Seyed Mohammad Ghodsi & Mohammadhossein Mirbolouk

  3. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Ahad Alizadeh

  4. Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Farah Bokharaei-Salim, Seyed Hamidreza Monavari & Hossein Keyvani

  5. Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

    Sedigheh Taghinezhad Saroukalaei

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  1. Farzin Sadeghi
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Correspondence to Hossein Keyvani.

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Sadeghi, F., Salehi-Vaziri, M., Ghodsi, S.M. et al. Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors. Arch Virol 160, 61–68 (2015). https://doi.org/10.1007/s00705-014-2230-0

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  • DOI: https://doi.org/10.1007/s00705-014-2230-0

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