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Analysis of small RNAs derived from Chinese wheat mosaic virus

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Abstract

The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21–22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5′-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted.

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Acknowledgments

This work was funded by the Special Fund for Agro-Scientific Research in the Public Interest of China (201303021), China Agriculture Research System (CARS-3-1) from the Ministry of Agriculture of China, Project of New Varieties of Genetically Modified Wheat of China (2011ZX08002-001), and the Zhejiang Provincial Foundation for Natural Science (Y3090657). We thank Professor M. J. Adams, Rothamsted Research, Harpenden, UK, for help in correcting the English of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Jian Yang, Shi-Ling Zheng, Heng-Mu Zhang, Xiao-Ya Liu, Jing Li, Jun-Min Li & Jian-Ping Chen

  2. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 304003, China

    Shi-Ling Zheng

  3. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China

    Xiao-Ya Liu

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  1. Jian Yang
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  2. Shi-Ling Zheng
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  3. Heng-Mu Zhang
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  5. Jing Li
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Corresponding authors

Correspondence to Heng-Mu Zhang or Jian-Ping Chen.

Additional information

J. Yang and S.-L. Zheng contributed equally to this work.

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705_2014_2155_MOESM1_ESM.jpg

Figure S1, Secondary structure prediction of the flanking sequences for the hotspots within the CWMV genomic RNA 1 (A). The number indicates the abundance of each corresponding position occurring in the vsiRNAs (JPEG 7194 kb)

705_2014_2155_MOESM2_ESM.jpg

Figure S1, Secondary structure prediction of the flanking sequences for the hotspots within the CWMV genomic RNA 2 (B). The number indicates the abundance of each corresponding position occurring in the vsiRNAs (JPEG 4195 kb)

Table S1, CWMV vsiRNA in a CWMV-infected plant (XLSX 1063 kb)

Table S2, The putative target genes of CWMV vsiRNA (XLSX 27 kb)

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Yang, J., Zheng, SL., Zhang, HM. et al. Analysis of small RNAs derived from Chinese wheat mosaic virus. Arch Virol 159, 3077–3082 (2014). https://doi.org/10.1007/s00705-014-2155-7

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  • DOI: https://doi.org/10.1007/s00705-014-2155-7

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