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Generic detection and identification of pospiviroids

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Abstract

A multiplex one-step RT-PCR aiming at detecting all pospiviroids known to be harmful to cultivated plants has been developed. Specificity, sensitivity, selectivity, repeatability and reproducibility of this test have been assessed in order to fulfill the recommendations of the EPPO standard PM7/98 and provide routine detection laboratories with a cost-effective, easy-to-use and robust pospiviroid detection test. To further understand the epidemiology and ease the management of pospiviroid outbreaks, this RT-PCR diagnostic test can be followed by direct sequencing of the amplicons to identify and characterize the detected pospiviroid isolates.

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Acknowledgements

We thank Alex Deterville, Daniel Goderniaux and Michel Facq for their indispensable help in the lab and in the greenhouse. We also thank our colleagues from the ANSES (France), Pascal Gentit, Xavier Tassus and Michèle Visage, as well as Dr. Francesco Faggioli from CRA-PAV (Italy) for their constructive discussions and for providing us with pospiviroids strains. This work was partially supported by the Federal Agency for the Safety of the Food Chain. The Walloon Agricultural Research Centre is financed by the Walloon Public Service of Agriculture, Environment and Tourism.

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Authors and Affiliations

  1. Walloon Agricultural Research Centre, 5030, Gembloux, Belgium

    Thibaut Olivier, Elisabeth Demonty, Frédéric Fauche & Stéphan Steyer

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  1. Thibaut Olivier
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  2. Elisabeth Demonty
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  3. Frédéric Fauche
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  4. Stéphan Steyer
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Correspondence to Thibaut Olivier.

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Olivier, T., Demonty, E., Fauche, F. et al. Generic detection and identification of pospiviroids. Arch Virol 159, 2097–2102 (2014). https://doi.org/10.1007/s00705-014-1978-6

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  • DOI: https://doi.org/10.1007/s00705-014-1978-6

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