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Molecular characterization of a rabies virus isolate from a rabid dog in Hanzhong District, Shaanxi Province, China

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Abstract

A canine rabies virus, Shaanxi-HZ-6, was isolated in Shaanxi Province, China, in 2009. Its genome has been completely sequenced and found to be closely related to the China I rabies virus strains widely circulating in China. The genomic length was 11,923 base pairs, and the overall organization of the genome was similar to that of other rabies virus isolates. Compared with isolates CQ92 and J, 84 amino acid substitutions (7 in the N gene, 15 in P, 6 in M, 25 in G, 31 in L) were observed in strain Shaanxi-HZ-6. Amino acid substitutions of R264H and V332I were noted in the G protein antigenic site I and site III, respectively. Residue 333 of the G protein, which is considered to be associated with pathogenicity, was Arg in Shaanxi-HZ-6. These and other substitutions may help provide an explanation why the China I lineage strain maintains its prevalence in China.

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Fig. 1
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Abbreviations

RT-PCR:

Reverse transcription polymerase chain reaction

IGRS:

Intergenic signals

References

  1. World Health Organization (2005) WHO Expert Committee on Rabies. 2004. First Report, WHO technical report series 931. World Health Organization, Geneva

    Google Scholar 

  2. Hu R, Tang Q, Tang J, Fooks AR (2009) Rabies in China: an update. Vector Borne Zoonotic Dis 9:1–12

    Article  PubMed  Google Scholar 

  3. Zhao J, Liu Y, Zhang S, Zhang F, Gao H, Hu R (2011) Analysis of an outbreak of human rabies in 2009 in Hanzhong District, Shaanxi Province, China. Vector Borne Zoonotic Dis 11:59–68

    Article  PubMed  Google Scholar 

  4. Zhao J, Zhang S, Liu Y, Zhang F, Hu R (2013) Complete genome sequence of a rabies virus isolate from a ferret badger (Melogale moschata) in Jiangxi, China. Genome Announc 1(3). doi:10.1128/genomeA.00192-13

  5. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Nadin-Davis SA, Huang W, Wandeler AI (1997) Polymorphism of rabies viruses within the phosphoprotein and matrix protein genes. Arch Virol 142:979–992

    Article  CAS  PubMed  Google Scholar 

  8. Nadin-Davis SA, Abdel-Malik M, Armstrong J, Wandeler AI (2002) Lyssavirus P gene characterisation provides insights into the phylogeny of the genus and identifies structural similarities and diversity within the encoded phosphoprotein. Virology 298:286–305

    Article  CAS  PubMed  Google Scholar 

  9. Jacob Y, Real E, Tordo N (2001) Functional interaction map of lyssavirus phosphoprotein: identification of the minimal transcription domains. J Virol 75:9613–9622

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Lo KW, Naisbitt S, Fan JS, Sheng M, Zhang M (2001) The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif. J Biol Chem 276:14059–14066

    Article  CAS  PubMed  Google Scholar 

  11. Raux H, Flamand A, Blondel D (2000) Interaction of the rabies virus P protein with the LC8 dynein light chain. J Virol 74:10212–10216

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Wirblich C, Tan GS, Papaneri A, Godlewski PJ, Orenstein JM et al (2008) PPEY motif within the rabies virus (RV) matrix protein is essential for efficient virion release and RV pathogenicity. J Virol 82:9730–9738

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Bourhy H, Kissi B, Tordo N (1993) Molecular diversity of the Lyssavirus genus. Virology 194:70–81

    Article  CAS  PubMed  Google Scholar 

  14. Lafon M (2005) Rabies virus receptors. J Neurovirol 11:82–87

    Article  CAS  PubMed  Google Scholar 

  15. Poch O, Blumberg BM, Bougueleret L, Tordo N (1990) Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol 71(Pt 5):1153–1162

    Article  CAS  PubMed  Google Scholar 

  16. Tordo N, Poch O, Ermine A, Keith G, Rougeon F (1988) Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology 165:565–576

    Article  CAS  PubMed  Google Scholar 

  17. Le Mercier P, Jacob Y, Tordo N (1997) The complete Mokola virus genome sequence: structure of the RNA-dependent RNA polymerase. J Gen Virol 78(Pt 7):1571–1576

    PubMed  Google Scholar 

  18. Chandrika R, Horikami SM, Smallwood S, Moyer SA (1995) Mutations in conserved domain I of the Sendai virus L polymerase protein uncouple transcription and replication. Virology 213:352–363

    Article  CAS  PubMed  Google Scholar 

  19. Yu J, Li H, Tang Q, Rayner S, Han N, Guo Z, Liu H, Adams J, Fang W, Tao X, Wang S, Liang G (2012) The spatial and temporal dynamics of rabies in China. PLOS Negl Trop Dis 6:e1640

    Article  PubMed Central  PubMed  Google Scholar 

  20. Guo Z, Tao X, Yin C, Han N, Yu J, Li H, Liu H, Fang W, Adams J, Wang J, Liang G, Tang Q, Rayner S (2013) National borders effectively halt the spread of rabies: the current rabies epidemic in China is dislocated from cases in neighboring countries. PLOS Negl Trop Dis 7:e2039

    Article  PubMed Central  PubMed  Google Scholar 

  21. Xie T, Yu H, Wu J, Ming P, Huang S, Shen Z, Xu G, Yan J, Yu B, Zhou D (2012) Molecular characterization of the complete genome of a street rabies virus WH11 isolated from donkey in China. Virus Genes 45:452–462

    Article  CAS  PubMed  Google Scholar 

  22. Tao X-Y, Tang Q, Rayner S, Guo Z-Y, Li H et al (2013) Molecular phylodynamic analysis indicates lineage displacement occurred in Chinese rabies epidemics between 1949 to 2010. PLOS Negl Trop Dis 7:e2294

    Article  PubMed Central  PubMed  Google Scholar 

  23. Hu R, Tang Q, Tang J, Fooks AR (2009) Rabies in China: an update. Vector Borne Zoonotic Dis 9(1):1–12

    Article  PubMed  Google Scholar 

  24. National Veterinary Medicine Foundation Information Query System (2012) http://sysjk.ivdc.gov.cn:8081/cx/. Jan. 1, 2012–Dec. 31, 2012

  25. Tang X, Luo M, Zhang S, Fooks AR, Hu R et al (2005) Pivotal role of dogs in rabies transmission, China. Emerg Infect Dis 11:1970–1972

    Article  PubMed Central  PubMed  Google Scholar 

  26. Coleman PG, Dye C (1996) Immunization coverage required to prevent outbreaks of dog rabies. Vaccine 14:185–186

    Article  CAS  PubMed  Google Scholar 

  27. Matsumoto T, Ahmed K, Wimalaratne O, Nanayakkara S, Perera D, Karunanayake D, Nishizono A (2011) Novel sylvatic rabies virus variant in endangered golden palm civet, Sri Lanka. Emerg Infect Dis 17:2346–2349

    Article  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the China National “973” Program (2005CB52300, 2011CB500705) and the National Natural Science Foundation of China (30630049, 30972199).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Laboratory of Epidemiology, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, 666 Liuying West Road, Changchun, 130122, Jilin, China

    Jinghui Zhao, Shuchao Wang, Shoufeng Zhang, Ye Liu, Jinxia Zhang, Fei Zhang, Lijuan Mi & Rongliang Hu

Authors
  1. Jinghui Zhao
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  2. Shuchao Wang
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  3. Shoufeng Zhang
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  4. Ye Liu
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  5. Jinxia Zhang
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  6. Fei Zhang
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  7. Lijuan Mi
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  8. Rongliang Hu
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Corresponding author

Correspondence to Rongliang Hu.

Additional information

J. Zhao, S. Wang, S. Zhang and Y. Liu contributed equally to this article.

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Zhao, J., Wang, S., Zhang, S. et al. Molecular characterization of a rabies virus isolate from a rabid dog in Hanzhong District, Shaanxi Province, China. Arch Virol 159, 1481–1486 (2014). https://doi.org/10.1007/s00705-013-1941-y

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  • DOI: https://doi.org/10.1007/s00705-013-1941-y

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