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Complete genome sequence of the original Taiwanese isolate of sweet potato latent virus and its relationship to other potyviruses infecting sweet potato

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Abstract

The complete genome of sweet potato latent virus (SPLV) was determined to be 10081 nucleotides long excluding the 3’ poly (A) tail. The genome contains a single large open reading frame encoding a polyprotein of 3247 amino acids. Its genomic organization is typical of potyviruses and contains motifs conserved in members of the genus Potyvirus. Pairwise comparisons show that SPLV shares identities of 50.0 %-56.3 % to other potyviruses at the genomic sequence level. Phylogenetic analysis shows that SPLV is closely related to four other sweet potato potyviruses in the sweet potato feathery mottle virus lineage, but it lacks the unique PISPO in the P1 region of those viruses. The genome analyses confirm that SPLV is a distinct sweet potato virus in the genus Potyvirus.

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Acknowledgments

The authors thank Crindi Loschinkohl, Whitney Hymes and Sam Grinstead for excellent technical assistance, and Dr. Gary Kinard for critical review of the manuscript.

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Authors and Affiliations

  1. USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Mingqiang Wang & Ruhui Li

  2. Plant Germplasm Quarantine Program, USDA-APHIS-PPQ-PHP, Beltsville, MD, 20705, USA

    Jorge Abad

  3. International Potato Center (CIP), Lima, Peru

    Segundo Fuentes

Authors
  1. Mingqiang Wang
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  2. Jorge Abad
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  3. Segundo Fuentes
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  4. Ruhui Li
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Correspondence to Ruhui Li.

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Wang, M., Abad, J., Fuentes, S. et al. Complete genome sequence of the original Taiwanese isolate of sweet potato latent virus and its relationship to other potyviruses infecting sweet potato. Arch Virol 158, 2189–2192 (2013). https://doi.org/10.1007/s00705-013-1705-8

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  • DOI: https://doi.org/10.1007/s00705-013-1705-8

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