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A variant of blueberry necrotic ring blotch virus associated with red lesions in blueberry

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Abstract

The complete genome of a variant of the multi-segmented (+) RNA virus blueberry necrotic ring blotch virus (BNRBV), which has not been assigned to a genus, was obtained from foliar red lesions on southern highbush blueberries grown in Alachua Co., Florida. The genome organization of this variant, BNRBV-RL, is the same as that of BNRBV: four genomic segments and seven ORFs (one ORF on each of RNA 1, RNA 2, and RNA 4 and as many as four ORFs on RNA 3). BLAST analysis revealed nucleic acid sequence identities of 89 %, 90 %, 90 % and 86 % to BNRBV RNA 1, RNA 2, RNA 3 and RNA 4, respectively. Phylogenetic analysis of the amino acid sequence of the putative RdRp domain indicated that BNRBV-RL was closely related to BNRBV and less related to citrus leprosis virus type C and three other mite-transmitted viruses. The nucleotide and amino acid sequence differences between BNRBV-RL and BNRBV combined with differences in symptom expression in blueberry would suggest that BNRBV-RL is a strain of BNRBV.

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Acknowledgments

This work was funded by the USDA-NIFA Specialty Crops Research Initiative Award No. 2008-51180-19579. We thank R.R. Martin for his collaboration.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Florida, Gainesville, FL, USA

    M. Cantu-Iris, P. F. Harmon, A. Londoño & J. E. Polston

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  1. M. Cantu-Iris
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  2. P. F. Harmon
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  3. A. Londoño
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  4. J. E. Polston
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Correspondence to J. E. Polston.

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Cantu-Iris, M., Harmon, P.F., Londoño, A. et al. A variant of blueberry necrotic ring blotch virus associated with red lesions in blueberry. Arch Virol 158, 2197–2200 (2013). https://doi.org/10.1007/s00705-013-1653-3

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  • DOI: https://doi.org/10.1007/s00705-013-1653-3

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