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A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia

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Abstract

A suspected virus disease was identified from an arborescent Brugmansia x candida Pers. (syn. Datura candida Pers.) tree. The causal agent was aphid transmissible at low rates. Viral particles were purified from infected tobacco tissue, analyzed, and purified virions were inoculated into healthy tobacco plants to recreate the symptoms. The virions had a mean length of 720-729 nm, and infected cells contained inclusion bodies typical of potyvirus infections. Analysis of infected tissues and purified virions with a panel of potyvirus-specific antibodies confirmed identification as a potyvirus. Viral host range, dilution end point, thermal tolerance and aphid transmission characteristics were examined. The viral genome (9761 nt) is typical of potyviruses, with the closest related potyvirus being pepper mottle virus, at 72 % nt sequence identity. Based on conventions for naming novel potyviruses, the virus was determined to be a member of a previously undescribed species, tentatively named “Brugmansia mosaic virus” (BruMV).

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Acknowledgments

The authors wish to acknowledge the contributions of Michelle Kuhlmann, Ann Echard, and Gene Milbrath for aspects of virus biology, M. Ken Corbett for excellent electron micrographs, and Robert Kahn for initial isolation and transmission of the virus.

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The use of trade, firm, or corporation names in this article is for the information and convenience of the reader. Such use does not constitute an official endorsement of approval by the USDA Agricultural Research Service, NAL or BIC of any product or service to the exclusion of others that may be suitable.

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Authors and Affiliations

  1. USDA-ARS Foreign Disease/Weed Science Research Unit, Fort Detrick, MD, USA

    Vernon D. Damsteegt, Andrew L. Stone, Diana J. Sherman & William L. Schneider

  2. Hood College, Frederick, USA

    Oney P. Smith

  3. American Type Culture Collection, Manassas, VA, USA

    Lawrence McDaniel

  4. USDA-ARS Appalachian Fruit Research Station, Kearneysville, WV, USA

    Chris Dardick

  5. USDA-ARS Floral and Nursery Plants Research Unit, Beltsville, MD, USA

    John Hammond & Ramon Jordan

Authors
  1. Vernon D. Damsteegt
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  2. Andrew L. Stone
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  3. Oney P. Smith
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  4. Lawrence McDaniel
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  5. Diana J. Sherman
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  6. Chris Dardick
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  7. John Hammond
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  8. Ramon Jordan
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  9. William L. Schneider
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Corresponding author

Correspondence to William L. Schneider.

Additional information

Lawrence McDaniel: Deceased.

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705_2012_1600_MOESM1_ESM.tif

Consistency of Brugmansia mosaic virus (BruMV) symptoms on Xanthi tobacco over the 40 years that the virus was maintained in the greenhouse. Panel A, BruMV on Xanthi tobacco in 1969; panel B, BruMV on Xanthi tobacco in 1974; panel C, BruMV on Xanthi tobacco in 1992; panel D, BruMV on Xanthi tobacco in 2012 (TIFF 299 kb)

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Damsteegt, V.D., Stone, A.L., Smith, O.P. et al. A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia . Arch Virol 158, 1235–1244 (2013). https://doi.org/10.1007/s00705-012-1600-8

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  • DOI: https://doi.org/10.1007/s00705-012-1600-8

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