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Molecular identification of enteroviruses associated with aseptic meningitis in children from India

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Abstract

We identified and characterized enteroviruses associated with aseptic meningitis in children between April 2009 and March 2010. Enterovirus RNA was detected in 51 (45.5 %) of 112 CSF samples. Molecular typing by RT-PCR and sequencing of a partial VP1 region revealed the predominance of echovirus (ECV) 32 (n = 20), followed by ECV 11 (n = 10), ECV 13 and ECV 14 (n = 5 each), coxsackievirus (CV) B3 and CV B6 (n = 3 each), CV A2, CV A10 and ECV 30 (n = 1 each). Phylogenetic analysis of ECV 32 showed 0 to 4 % sequence divergence among strains of the present study and 20-23 % from the prototype Puerto Rico strain at the nucleotide level. This is the first report of ECV 32 associated with an aseptic meningitis epidemic and identification of seven different enterovirus serotypes (CV A2, CV A10, CV B3, CV B6, ECV 13, ECV 14 and ECV 32) in meningitis cases from India.

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Fig. 1

Abbreviations

EV:

Enterovirus

ECV:

Echovirus

CV:

Coxsackie virus

CSF:

Cerebrospinal fluid

RT-PCR:

Reverse transcriptase polymerase chain reaction

NCR:

Non-coding region

RD:

Human rhabdomyosarcoma cells

HEp 2:

Human epithelium larynx

CPE:

Cytopathic effect

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Acknowledgments

This study was supported by a grant-in-aid from Indian Council of Medical Research, Government of India, New Delhi (ref. no: 5/8/7/23/2007-ECD-I).

Conflict of interest

The authors declare no conflict of interests.

Ethical approval

This study was approved by the Institutional Ethics Committee, Chhatrapati Shauji Maharaj Medical University, Lucknow (ref. no. XXXVII-ECM/A-P12), and Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow (A-04 PGI/EMP/IEC/46/25.07.2009), Uttar Pradesh, India.

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Authors and Affiliations

  1. Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226014, Uttar Pradesh, India

    Arvind Kumar, Deepti Shukla & Tapan N. Dhole

  2. Department of Paediatrics, Chhatrapati Shahuji Maharaj Medical University, Lucknow, 226003, Uttar Pradesh, India

    Rashmi Kumar & Prashant Jauhari

  3. Department of Community Medicine, Chhatrapati Shahuji Maharaj Medical University, Lucknow, 226003, Uttar Pradesh, India

    Arvind Kumar & Mohammad Z. Idris

  4. Department of Botany, Lucknow University, Lucknow, 226007, Uttar Pradesh, India

    Deepti Shukla & Shalini Srivastava

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  1. Arvind Kumar
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  2. Deepti Shukla
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  7. Tapan N. Dhole
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Correspondence to Tapan N. Dhole.

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Kumar, A., Shukla, D., Kumar, R. et al. Molecular identification of enteroviruses associated with aseptic meningitis in children from India. Arch Virol 158, 211–215 (2013). https://doi.org/10.1007/s00705-012-1476-7

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