Abstract
A quasi-spherical virus was isolated from a cultivated Amazon lily plant (Eucharis grandiflora) that could be mechanically transmitted to healthy E. grandiflora plants, subsequently producing mild mosaic or mottle symptoms on the leaves. The purified virus consisted of three quasi-spherical particles about 20 nm wide and 70, 40 and 30 nm in length, containing three segmented genomes of 3,169, 2,507 and 2,530 nucleotides, respectively. Sequence analysis showed that the newly isolated virus is related to pelargonium zonate spot virus, a member of the genus Anulavirus. We propose that the virus should be designated as Amazon lily mild mottle virus (ALiMMV).
References
Alexandre MAV, Duarte LML, Rivas EB, Cilli A, Harakava R, Galleti SR, Kitajima EW (2011) Hippeastrum mosaic virus diagnosed in Hippeastrum and Eucharis in Brazil. J Plant Pathol 93:643–649
Finetti-Sialer M, Gallitelli D (2003) Complete nucleotide sequence of Pelargonium zonate spot virus and its relationship with the family Bromoviridae. J Gen Virol 84:3143–3151
Gallitelli D (1982) Properties of a tomato isolate of Pelargonium zonate spot virus. Ann Appl Biol 100:457–466
Gallitelli D, Finetti-Sialer M, Martelli GP (2005) Anulavirus, a proposed new genus of plant viruses in the family Bromoviridae. Arch Virol 150:407–411
Jayasinghe U, Dijkstra J (1979) Hippeastrum mosaic virus and another filamentous virus in Eucharis grandiflora. Neth J Plant Pathol 85:47–65
King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2011) Virus taxonomy: In 9th report of the international committee on taxonomy of viruses. Elsevier Academic Press, Amsterdam
Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequence. Crit Rev Biochem Mol Biol 28:546
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lapidot M, Guenoume-Gelbart D, Leibman D, Holdengreber V, Davidovitz M, Machbash Z, Klieman-Shoval S, Choen S, Gal-On A (2010) Pelargonium zonate spot virus is transmitted vertically via seed and pollen in Tomato. Phytopathology 100:798–804
Melcher U (2000) The ‘30K’ superfamily of viral movement proteins. J Gen Virol 81:257–266
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor laboratory Press, New York
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Terami F, Honda Y, Fukumoto F (1995) Amazon lily mosaic virus, a new potyvirus infecting Amazon lily (Eucharis grandiflora). Ann Phytopathol Soc Jpn 61:1–6
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fuji, S., Kikuchi, M., Ueda, S. et al. Characterization of a new anulavirus isolated from Amazon lily plants. Arch Virol 158, 201–206 (2013). https://doi.org/10.1007/s00705-012-1467-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-012-1467-8