Abstract
Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2*0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.
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Acknowledgments
We thank Dr. Sandra Perez for her helpful advice in the proofreading of this manuscript. The authors also thank Patricia Bani and Norma Rodriguez for technical assistance. This work has been partially supported by Consejo Nacional Investigaciones Cientificas y Tecnologicas (PIP 577) and Secretaria Ciencia, Arte y Tecnologia, Universidad Nacional del Centro de la Provincia de Buenos Aires.
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M. A. Juliarena and P. A. Lendez contributed equally to the development of this work.
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Juliarena, M.A., Lendez, P.A., Gutierrez, S.E. et al. Partial molecular characterization of different proviral strains of bovine leukemia virus. Arch Virol 158, 63–70 (2013). https://doi.org/10.1007/s00705-012-1459-8
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DOI: https://doi.org/10.1007/s00705-012-1459-8