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Detection of human bocaviruses 1, 2 and 3 in Irish children presenting with gastroenteritis

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Abstract

Human bocavirus has been increasingly detected worldwide in patients suffering from gastroenteritis, with the highest incidence reported in children aged between 6 and 24 months. A total of 155 non-bacterial gastroenteritis samples consisting of rotavirus-, adenovirus- and norovirus-positive specimens were collected from patients in the Munster region of Ireland from 2006-2008. Of these 155, a total of 12 were positive for the presence of human bocavirus. Three types of human bocavirus were identified: HBoV1, 2 and 3. In addition, recombinant strains (n=4) were also isolated. This is the first report of all three types of HBoV being detected in southern Ireland.

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References

  1. Marie-Cardine A, Gourlain K, Mouterde O, Castignolles N, Hellot MF, Mallet E et al (2002) Epidemiology of acute viral gastroenteritis in children hospitalized in Rouen, France. Clin Infect Dis 34:1170–1178

    Article  PubMed  Google Scholar 

  2. Lennon G, Cashman O, Lane K, Cryan B, O’Shea H (2007) Prevalence and characterization of enteric adenoviruses in the South of Ireland. J Med Virol 79:1518–1526

    Article  PubMed  CAS  Google Scholar 

  3. Lau SK, Yip CC, Que TL, Lee RA, Au-Yeung RK, Zhou B et al (2007) Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis 196:986–993

    Article  PubMed  CAS  Google Scholar 

  4. Lee JI, Chung JY, Han TH, Song MO, Hwang ES (2007) Detection of human bocavirus in children hospitalized because of acute gastroenteritis. J Infect Dis 196:994–997

    Article  PubMed  Google Scholar 

  5. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B (2005) Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 102:12891–12896

    Article  PubMed  CAS  Google Scholar 

  6. Sun Y, Chen AY, Cheng F, Guan W, Johnson FB, Qiu J (2009) Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. Virol 83:3956–3967

    Article  CAS  Google Scholar 

  7. Schwartz D, Green B, Carmichael LE, Parrish CR (2002) The canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus. Virol 302:219–223

    Article  CAS  Google Scholar 

  8. Moffatt S, Yaegashi N, Tada K, Tanaka N, Sugamura K (1998) Human parvovirus B19 nonstructural (NS1) protein induces apoptosis in erythroid lineage cells. J Virol 72:3018–3028

    PubMed  CAS  Google Scholar 

  9. Schildgen O, Müller A, Allander T, Mackay IM, Völz S, Kupfer B et al (2008) Human bocavirus: passenger or pathogen in acute respiratory tract infections? Clin Microbiol Rev 21:291–304

    Article  PubMed  CAS  Google Scholar 

  10. Lindner J, Karalar L, Schimanski S, Pfister H, Struff W, Modrow S (2008) Clinical and epidemiological aspects of human bocavirus infection. J Clin Virol 43:391–395

    Article  PubMed  CAS  Google Scholar 

  11. Lüsebrink J, Wittleben F, Schildgen V, Schildgen O (2009) Human bocavirus—insights into a newly identified respiratory virus. Viruses 1:3–12

    Article  PubMed  Google Scholar 

  12. Kleines M, Scheithauer S, Rackowitz A, Ritter K, Häusler M (2007) High prevalence of human bocavirus detected in young children with severe acute lower respiratory tract disease by use of a standard PCR protocol and a novel real-time PCR protocol. J Clin Microbiol 45:1032–1034

    Article  PubMed  CAS  Google Scholar 

  13. Song JR, Jin Y, Xie ZP, Gao HC, Xiao NG, Chen WX et al (2010) Novel human bocavirus in children with acute respiratory tract infection. Emerg Infect Dis 16:324–327

    Article  PubMed  Google Scholar 

  14. Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L, Sethabutr O et al (2010) Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 201:1633–1643

    Article  PubMed  CAS  Google Scholar 

  15. Kapoor A, Slikas E, Simmonds P, Chieochansin T, Naeem A, Shaukat S et al (2009) A newly identified bocavirus species in human stool. J Infect Dis 199:196–200

    Article  PubMed  Google Scholar 

  16. Arden KE, McErlean P, Nissen MD, Sloots TP, Mackay IM (2006) Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. J Med Virol 78:1232–1240

    Article  PubMed  CAS  Google Scholar 

  17. Manning A, Russell V, Eastick K, Leadbetter GH, Hallam N, Templeton K, Simmonds P (2006) Epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. J Infect Dis 194:1283–1290

    Article  PubMed  CAS  Google Scholar 

  18. Bastien N, Brandt K, Dust K, Ward D, Li Y (2006) Human bocavirus infection, Canada. Emerg Infect Dis 12:848–850

    Article  PubMed  Google Scholar 

  19. Binn LN, Lazar EC, Eddy GA, Kajima M (1970) Recovery and characterization of a minute virus of canines. Infect Immun 1:503–508

    PubMed  CAS  Google Scholar 

  20. Storz J, Leary JJ, Carlson JH, Bates RC (1978) Parvoviruses associated with diarrhea in calves. J Am Vet Med Assoc 173:624–627

    PubMed  CAS  Google Scholar 

  21. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  22. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  23. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035

    Article  PubMed  CAS  Google Scholar 

  24. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  25. Cashman O, Collins PJ, Lennon G, Cryan B, Martella V, Fanning S et al (2011) Molecular characterisation of group a rotaviruses detected in children with gastroenteritis in Ireland in the years 2006–2009. Epidemiol Infect 14:1–13

    Google Scholar 

  26. Shackelton LA, Hoelzer K, Parrish CR, Holmes EC (2007) Comparative analysis reveals frequent recombination in the parvoviruses. J Gen Virol 88:3294–3301

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

Funding for this research was provided through the Food Institutional Research Measure, (grant no. 05/R&D/CIT/365, awarded to HO’S), administered by the Department of Agriculture, Fisheries & Food, Ireland. Special thanks to Dr. Tobias Allander and Dr. Juha Lindner for bocavirus controls.

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None declared.

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  1. Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Ireland

    O. Cashman & H. O’Shea

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  1. O. Cashman
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  2. H. O’Shea
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Correspondence to H. O’Shea.

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Cashman, O., O’Shea, H. Detection of human bocaviruses 1, 2 and 3 in Irish children presenting with gastroenteritis. Arch Virol 157, 1767–1773 (2012). https://doi.org/10.1007/s00705-012-1343-6

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  • DOI: https://doi.org/10.1007/s00705-012-1343-6

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