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Identification of a new genotype of bovine leukemia virus

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Abstract

To investigate the degree of genetic variability of bovine leukemia virus (BLV) strains circulating in Croatia, 29 isolates from the six largest dairy farms were examined by PCR for a segment of the gp51 env gene, followed by DNA sequencing and phylogenetic analysis. The nucleotide sequences were compared with other previously characterized BLV strains from different geographical areas, comprising all seven known BLV genotypes. The Croatian sequences showed six to eight nucleotide substitutions: six silent substitutions and two amino acid changes. Four of those substitutions were within epitopes. In comparison to the sequences of other BLV genotypes, our isolates showed the closest relationship to genotype 1 isolates PL-3252 (FJ808585) and AL-148 (FJ808573) from Argentina. The degree of variation between our sequences and those of genotype 1 was 0.2- 4.6 %. In phylogenetic trees based on 400-nt and 519-nt sequences, all of the Croatian sequences clustered separately from the other sequences, revealing a new genotype.

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Acknowledgments

This research was supported by grant no. 048-0481186-1183 from the Ministry of Science, Education and Sports, Republic of Croatia. The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Veterinary Department Vinkovci, Croatian Veterinary Institute, Vinkovci, Croatia

    Davor Balić & Marin Periškić

  2. Department of Virology, Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia

    Ivana Lojkić, Tomislav Bedeković, Andreja Jungić, Nina Lemo, Besi Roić & Željko Čač

  3. Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

    Ljubo Barbić & Josip Madić

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  1. Davor Balić
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  2. Ivana Lojkić
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Correspondence to Ivana Lojkić.

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Balić, D., Lojkić, I., Periškić, M. et al. Identification of a new genotype of bovine leukemia virus. Arch Virol 157, 1281–1290 (2012). https://doi.org/10.1007/s00705-012-1300-4

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  • DOI: https://doi.org/10.1007/s00705-012-1300-4

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