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Genomic characterization of a cell-culture-adapted Korean human G9P[8] rotavirus, CAU05-202

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Abstract

The human rotavirus G9 strain is the fifth most common rotavirus worldwide. A human rotavirus G9P[8] strain CAU05-202 was isolated from a young child with diarrhea using a cell culture system, and its major gene sequences were determined. Phylogenetic analysis of the VP7 gene revealed that CAU05-202 clustered into genetic lineage III-d and was most closely related to G9 rotaviruses from Turkey (strain GUH13) and Sri Lanka (strain 05SLC056 and 05SLC057). VP4 and NSP4 gene analysis showed that CAU05-202 belongs to the P[8]-3 lineage and genotype B, respectively. In addition, CAU05-202 has a long RNA electropherotype, supported by VP6 gene analysis, which is clearly associated with subgroup II specificity. Analysis of the G9 rotavirus strain CAU05-202 provides information concerning the genetic relationships among global rotavirus G9 strains, suggesting that closely related G9 strains are persistent and widespread in Asian countries.

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Acknowledgments

This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea (A085138).

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Author notes

  1. Van Phan Le

    Present address: Faculty of Veterinary Medicine, Hanoi Agricultural University, Hanoi, Vietnam

Authors and Affiliations

  1. Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea

    Jong Wook Shin

  2. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea

    Van Phan Le, Van Thai Than, Yoosik Yoon, Kijeong Kim, Sang-In Chung & Wonyong Kim

  3. Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, South Korea

    Inseok Lim

  4. Department of Urology, College of Medicine, Chung-Ang University, Seoul, South Korea

    Soon Chul Myung

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  1. Jong Wook Shin
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Correspondence to Wonyong Kim.

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Shin, J.W., Le, V.P., Than, V.T. et al. Genomic characterization of a cell-culture-adapted Korean human G9P[8] rotavirus, CAU05-202. Arch Virol 157, 753–759 (2012). https://doi.org/10.1007/s00705-012-1232-z

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  • DOI: https://doi.org/10.1007/s00705-012-1232-z

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