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Pepper necrotic spot virus, a new tospovirus infecting solanaceous crops in Peru

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Abstract

Two virus isolates, T1 and T2, causing necrotic spots on leaves and stems of pepper and tomato, respectively, were isolated in the La Joya valley, Arequipa, Peru, in 2010. These two isolates were inoculated to differential hosts for tospoviruses and showed differential fitness: T1 induced necrotic local lesions in Vigna unguiculata, whereas T2 produced only chlorotic spots. The complete nucleotide sequence of the small (S) RNA from T2 and 1863 bp of the S RNA from T1 were determined. The deduced N protein sequence showed high amino acid identity (97%) between the isolates, indicating that the T1 and T2 are isolates of the same virus. Sequence comparisons indicated that the amino acid sequence of the N protein shared 53.49-87.98% identity with known American tospoviruses. Phylogenetic analysis of both the NSs and N proteins revealed that this new tospovirus belongs to the American group. We conclude that this tospovirus should be considered a member of a new species. The name Pepper necrotic spot virus (PNSV) is proposed.

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Acknowledgments

The authors wish to thank Dr. H. Pappu for his critical review of the manuscript and A. Redondo and I. de la Peña for technical assistance. This work was supported by Ministerio de Ciencia e Innovación (grant RTA2009-00081), cofinanced by FEDER (European Fund for Regional Development). R. Torres was the recipient of funds from CONCYTEC (Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica) during his stay in Madrid.

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Authors and Affiliations

  1. Departamento de Protección Vegetal, INIA, Carretera de La Coruña km. 7.0, 28040, Madrid, Spain

    Roger Torres, Javiera Larenas & Javier Romero

  2. Departamento de Fitopatología, Universidad Nacional Agraria La Molina (UNALM), Lima, Perú

    Roger Torres & Cesar Fribourg

Authors
  1. Roger Torres
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  2. Javiera Larenas
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  3. Cesar Fribourg
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  4. Javier Romero
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Corresponding author

Correspondence to Javier Romero.

Additional information

The nucleotide sequence data reported in this manuscript have been deposited in the DDBJ/EMBL/GenBank under accession numbers HE584762 and HE584763.

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Torres, R., Larenas, J., Fribourg, C. et al. Pepper necrotic spot virus, a new tospovirus infecting solanaceous crops in Peru. Arch Virol 157, 609–615 (2012). https://doi.org/10.1007/s00705-011-1217-3

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  • DOI: https://doi.org/10.1007/s00705-011-1217-3

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